Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120, People's Republic of China.
Inflammation. 2013 Dec;36(6):1415-23. doi: 10.1007/s10753-013-9681-4.
Recent reports suggest that src suppressed c kinase substrates (SSeCKS) are early inflammatory response protein. However, there is only scarce knowledge on the functional role of SSeCKS in liver under conditions of acute inflammation. In the present study, we investigated SSeCKS expression in liver after administration of carbon tetrachloride (CCl4) in rats and in isolated primary hepatic stellate cells (HSCs) upon activation on a plastic dish. We found that SSeCKS mRNA was hardly detectable in healthy liver tissue and further increased in carbon tetrachloride-mediated acute liver failure. SSeCKS protein expression was mainly found in hepatic stellate cells. In vitro, SSeCKS expression in activated rat HSCs was dramatically increased. The upregulation of SSeCKS protein expression in rat HSCs during activation in vitro and in vivo suggested the possibility of SSeCKS, an important part of function of the activated HSCs, perhaps through modulation of liver regeneration or formation of liver fibrosis after various injuries.
最近的报告表明，src 抑制性 c 激酶底物 (SSeCKS) 是早期炎症反应蛋白。然而，关于 SSeCKS 在急性炎症条件下肝脏中的功能作用的知识还很有限。在本研究中，我们研究了四氯化碳 (CCl4) 给药后大鼠肝脏中 SSeCKS 的表达情况，以及在塑料培养皿上激活时分离的原代肝星状细胞 (HSCs) 中 SSeCKS 的表达情况。我们发现 SSeCKS mRNA 在健康的肝组织中几乎检测不到，而在四氯化碳介导的急性肝衰竭中则进一步增加。SSeCKS 蛋白表达主要在肝星状细胞中发现。在体外，激活的大鼠 HSCs 中的 SSeCKS 表达显著增加。体外和体内激活过程中大鼠 HSCs 中 SSeCKS 蛋白表达的上调表明，SSeCKS 可能是激活的 HSCs 功能的重要组成部分，也许是通过调节各种损伤后的肝再生或肝纤维化形成。
