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通过沿着噬菌体表面组装组合抗体Fab文库将识别与复制功能联系起来。

Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces.

作者信息

Kang A S, Barbas C F, Janda K D, Benkovic S J, Lerner R A

机构信息

Department of Molecular Biology Scripps Research Institute, La Jolla, CA 92037.

出版信息

Proc Natl Acad Sci U S A. 1991 May 15;88(10):4363-6. doi: 10.1073/pnas.88.10.4363.

DOI:10.1073/pnas.88.10.4363
PMID:1903540
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC51659/
Abstract

We describe a method based on a phagemid vector with helper phage rescue for the construction and rapid analysis of combinatorial antibody Fab libraries. This approach should allow the generation and selection of many monoclonal antibodies. Antibody genes are expressed in concert with phage morphogenesis, thereby allowing incorporation of functional Fab molecules along the surface of filamentous phage. The power of the method depends upon the linkage of recognition and replication functions and is not limited to antibody molecules.

摘要

我们描述了一种基于噬菌粒载体并借助辅助噬菌体拯救的方法，用于构建和快速分析组合抗体Fab文库。这种方法应能实现多种单克隆抗体的产生和筛选。抗体基因与噬菌体形态发生协同表达，从而使功能性Fab分子沿丝状噬菌体表面掺入。该方法的强大之处在于识别功能与复制功能的联系，且不限于抗体分子。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/cb2c9b76791f/pnas01060-0315-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/49d6086b529a/pnas01060-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/3ec19b7d2baa/pnas01060-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/cb2c9b76791f/pnas01060-0315-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/49d6086b529a/pnas01060-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/3ec19b7d2baa/pnas01060-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/04f9/51659/cb2c9b76791f/pnas01060-0315-b.jpg

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