Better M, Chang C P, Robinson R R, Horwitz A H
International Genetic Engineering Inc. (INGENE), Santa Monica, CA 90404.
Science. 1988 May 20;240(4855):1041-3. doi: 10.1126/science.3285471.
A chimeric mouse-human Fab protein that binds specifically to the human carcinoma cell line C3347 has been expressed and secreted from Escherichia coli. This molecule, which contains functionally assembled kappa and Fd proteins, binds as effectively to sites on the surface of C3347 cells as Fab fragments prepared proteolytically from whole chimeric or mouse antibody. The production in Escherichia coli of foreign heterodimeric protein reagents, such as Fab, should prove useful in the management of human disease.
一种能特异性结合人癌细胞系C3347的嵌合鼠-人Fab蛋白已在大肠杆菌中表达并分泌。该分子包含功能组装的κ和Fd蛋白，其与C3347细胞表面位点的结合效果与从完整嵌合抗体或鼠抗体经蛋白酶解制备的Fab片段相同。在大肠杆菌中生产诸如Fab等外源异二聚体蛋白试剂，在人类疾病治疗中应会证明有用。
