Décordé Kelly, Teissèdre Pierre-Louis, Sutra Thibault, Ventura Emilie, Cristol Jean-Paul, Rouanet Jean-Max
Equipe d'Accueil 4188, Nutrition Humaine, Biodisponibilité et Athérogénèse, Université Montpellier 1 and 2, Montpellier, France.
Mol Nutr Food Res. 2009 May;53(5):659-66. doi: 10.1002/mnfr.200800165.
Studies reported the effects of polyphenols but not for grape polyphenols towards obesity. We analysed the effects of a polyphenolic grape seed extract (GSE) on obesity and oxidative stress in hamsters receiving a high-fat diet (HFD). Three groups of hamsters received a standard diet (STD), or a HFD plus a daily gavage with water (Control, HFD) or a solution of GSE (HFD + GSE) for 12 wk. Plasma glucose, triglycerides (TG), insulin, leptin and adiponectin were measured. Oxidative stress was assessed by cardiac production of superoxide anion and NAD(P)H oxidase expression. After 12 wk, HFD increased abdominal fat as compared with standards. GSE avoided this feature. HFD led to higher plasma glucose, TG, insulin and greater insulin resistance (HOMA-IR) values. GSE prevented in part these effects, reducing insulinemia and leptinemia by 16.5 and 45%, respectively, whereas adiponectin level increased by 61% compared with obese controls. GSE lowered glycemia and HOMA-IR and strongly prevented cardiac production of superoxide by 74% and NAD(P)H oxidase expression by 30%. This is the first time that chronic consumption of grape phenolics is shown to reduce obesity development and related metabolic pathways including adipokine secretion and oxidative stress.
已有研究报道了多酚类物质的作用，但未涉及葡萄多酚对肥胖的影响。我们分析了一种多酚类葡萄籽提取物（GSE）对高脂饮食（HFD）仓鼠肥胖及氧化应激的影响。三组仓鼠分别接受标准饮食（STD）、高脂饮食加每日灌胃水（对照组，HFD）或GSE溶液（HFD + GSE），持续12周。检测血浆葡萄糖、甘油三酯（TG）、胰岛素、瘦素和脂联素水平。通过心脏中超氧阴离子的产生及NAD(P)H氧化酶的表达评估氧化应激。12周后，与标准饮食组相比，高脂饮食组腹部脂肪增加。GSE可避免这一现象。高脂饮食导致血浆葡萄糖、TG、胰岛素水平升高及胰岛素抵抗（HOMA-IR）值增大。GSE部分预防了这些影响，使胰岛素血症和瘦素血症分别降低了16.5%和45%，而与肥胖对照组相比，脂联素水平升高了61%。GSE降低了血糖和HOMA-IR，并显著抑制了心脏中超氧阴离子生成达74%，NAD(P)H氧化酶表达降低30%。这是首次表明长期食用葡萄酚类物质可减少肥胖的发生及相关代谢途径，包括脂肪因子分泌和氧化应激。
