Koole Rolf, van Schooneveld Matti M, Hilhorst Jan, Castermans Karolien, Cormode David P, Strijkers Gustav J, de Mello Donegá Celso, Vanmaekelbergh Daniel, Griffioen Arjan W, Nicolay Klaas, Fayad Zahi A, Meijerink Andries, Mulder Willem J M
Condensed Matter and Interfaces, University Utrecht, P.O. Box 80000, 3508 TA Utrecht, The Netherlands.
Bioconjug Chem. 2008 Dec;19(12):2471-9. doi: 10.1021/bc800368x.
Silica particles as a nanoparticulate carrier material for contrast agents have received considerable attention the past few years, since the material holds great promise for biomedical applications. A key feature for successful application of this material in vivo is biocompatibility, which may be significantly improved by appropriate surface modification. In this study, we report a novel strategy to coat silica particles with a dense monolayer of paramagnetic and PEGylated lipids. The silica nanoparticles carry a quantum dot in their center and are made target-specific by the conjugation of multiple alphavbeta3-integrin-specific RGD-peptides. We demonstrate their specific uptake by endothelial cells in vitro using fluorescence microscopy, quantitative fluorescence imaging, and magnetic resonance imaging. The lipid-coated silica particles introduced here represent a new platform for nanoparticulate multimodality contrast agents.
在过去几年中，二氧化硅颗粒作为造影剂的纳米颗粒载体材料受到了广泛关注，因为这种材料在生物医学应用方面具有巨大潜力。该材料在体内成功应用的一个关键特性是生物相容性，通过适当的表面修饰可以显著提高其生物相容性。在本研究中，我们报告了一种用致密单层顺磁性和聚乙二醇化脂质包覆二氧化硅颗粒的新策略。二氧化硅纳米颗粒在其中心携带一个量子点，并通过多个αvβ3整合素特异性RGD肽的缀合使其具有靶向特异性。我们使用荧光显微镜、定量荧光成像和磁共振成像在体外证明了它们被内皮细胞的特异性摄取。这里引入的脂质包覆二氧化硅颗粒代表了一种用于纳米颗粒多模态造影剂的新平台。
