Acsadi G, Jiao S S, Jani A, Duke D, Williams P, Chong W, Wolff J A
Department of Pediatrics, Waisman Center, University of Wisconsin, Madison 53706.
New Biol. 1991 Jan;3(1):71-81.
We found previously that genes injected into skeletal muscle can be taken up by myofibers and expressed. In the present study we found that myocardial cells can also express a variety of reporter genes injected into myocardium as efficiently as skeletal myofibers, while the cells of several other tissues cannot. The inability of tissues other than striated muscle to express injected DNA is not due to technical difficulties of injection because injected DNA was detected in these other tissues by PCR analysis. These results suggest that skeletal and cardiac muscle cells have unique features such as T tubules that may play a critical role in DNA uptake. Expression in cardiac muscle was stable for only two weeks, possibly because of an immune response against the transfected cells. The ability to directly transfer genes into myocardial cells raises the possibility of gene therapy for both acquired and genetic heart diseases.
我们先前发现，注入骨骼肌的基因可被肌纤维摄取并表达。在本研究中，我们发现心肌细胞也能高效表达注入心肌的多种报告基因，其效率与骨骼肌纤维相同，而其他几种组织的细胞则不能。除横纹肌外的其他组织无法表达注入的DNA，并非由于注射技术困难，因为通过PCR分析在这些其他组织中检测到了注入的DNA。这些结果表明，骨骼肌和心肌细胞具有诸如T小管等独特特征，这些特征可能在DNA摄取中起关键作用。心肌中的表达仅稳定两周，可能是因为对转染细胞产生了免疫反应。直接将基因导入心肌细胞的能力为获得性和遗传性心脏病的基因治疗带来了可能性。
