Lu Huijun, Huo Xiaowei, Zhang Ying, Zheng Min, Ma Mingxiao, Zhang Hongyong, Jin Minglan, Shen Guoshun, Jia Leili, Ji Yue, Li Xu, Jin Kuoshi, Jin Ningyi
Genetic Engineering Laboratory, Institute of Military Veterinary, Academy of Military Medical Sciences, China.
Microbiol Immunol. 2008 Sep;52(9):440-6. doi: 10.1111/j.1348-0421.2008.00057.x.
DNA-based vaccination is an attractive alternative for overcoming the disadvantages of inactivated virus vaccines; however, DNA vaccines alone often generate only weak immune responses. In this study, the efficacy of LMS as a chemical adjuvant on a DNA vaccine (pVIR-P12A-IL18-3C) encoding the P1-2A and 3C genes of the FMDV and swine IL-18, which provides protection against FMDV challenge, was tested. All test pigs were administered booster vaccinations 28 days after the initial inoculation, and were challenged with 1000 ID50 FMDV O/NY00 20 days after the booster vaccination. Positive and negative control groups were inoculated with inactivated virus vaccine and PBS respectively. The DNA vaccine plus LMS induced greater humoral and cell-mediated responses than the DNA vaccine alone, as evidenced by higher concentrations of neutralizing and specific anti-FMDV antibodies, and by higher concentrations of T-lymphocyte proliferation and IFN-y production, respectively. FMDV challenge revealed that the DNA vaccine plus LMS provided higher protection than the DNA vaccine alone. This study demonstrates that LMS may be useful as an adjuvant for improving the protective efficiency of DNA vaccination against FMDV in pigs.
基于DNA的疫苗接种是克服灭活病毒疫苗缺点的一种有吸引力的替代方法；然而，单独的DNA疫苗通常只能产生微弱的免疫反应。在本研究中，测试了LMS作为化学佐剂对编码口蹄疫病毒（FMDV）的P1-2A和3C基因以及猪白细胞介素-18（IL-18）的DNA疫苗（pVIR-P12A-IL18-3C）的效果，该疫苗可提供针对FMDV攻击的保护。所有试验猪在初次接种后28天进行加强免疫，并在加强免疫后20天用1000 ID50 FMDV O/NY00进行攻毒。阳性和阴性对照组分别接种灭活病毒疫苗和PBS。与单独的DNA疫苗相比，DNA疫苗加LMS诱导了更强的体液和细胞介导反应，分别表现为更高浓度的中和抗体和特异性抗FMDV抗体，以及更高浓度的T淋巴细胞增殖和IFN-γ产生。FMDV攻毒显示，DNA疫苗加LMS比单独的DNA疫苗提供了更高的保护。本研究表明，LMS作为佐剂可能有助于提高猪DNA疫苗接种对FMDV的保护效率。
