Pérez-Laínez Dolores, García-Mateos Rosario, San Miguel-Chávez Ruben, Soto-Hernández Marcos, Rodríguez-Pérez Enrique, Kite Geofrey
Departamento de Fitotecnia, Universidad Autónoma Chapingo, Chapingo, México, 56230, México.
Z Naturforsch C J Biosci. 2008 Sep-Oct;63(9-10):653-7. doi: 10.1515/znc-2008-9-1007.
Calia secundiflora (Ortega) Yakovlev (Fabaceae) is considered a medicinal plant in Mexico but has scarcely been used because of the toxicity of its quinolizidine alkaloids. Several quinolizidine alkaloids have shown bactericidal, nematicidal, and fungicidal activities. The purpose of this study was to identify the alkaloids in the seeds and evaluate the activity of the organic extract on several phytopathogenic fungi and bacteria. An in vitro bioassay was conducted with species of the following phytopathogenic fungi: Alternaria solani, Fusarium oxysporum and Monilia fructicola; and of the following bacteria Pseudomonas sp., Xanthomonas campestris and Erwinia carotovora. Cytisine, lupinine, anagyrine, sparteine, N-methylcytisine, 5,6-dehydrolupanine, and lupanine were identified by liquid chromatography-mass spectrometry in the extract of seeds; the most abundant compound of the extract was cytisine. It was observed that the crude extract of Calia secundiflora was moderately active on bacteria and more potent on phytopathogenic fungi. In contrast cytisine showed the opposite effects.
二花卡利亚豆（Calia secundiflora (Ortega) Yakovlev，豆科）在墨西哥被视为一种药用植物，但由于其喹诺里西啶生物碱的毒性，几乎未被使用。几种喹诺里西啶生物碱已显示出杀菌、杀线虫和杀真菌活性。本研究的目的是鉴定种子中的生物碱，并评估有机提取物对几种植物病原真菌和细菌的活性。对以下植物病原真菌进行了体外生物测定：茄链格孢（Alternaria solani）、尖孢镰刀菌（Fusarium oxysporum）和褐腐病菌（Monilia fructicola）；以及以下细菌：假单胞菌属（Pseudomonas sp.）、野油菜黄单胞菌（Xanthomonas campestris）和胡萝卜软腐欧文氏菌（Erwinia carotovora）。通过液相色谱-质谱法在种子提取物中鉴定出了金雀花碱、羽扇豆碱、臭豆碱、鹰爪豆碱、N-甲基金雀花碱、5,6-脱氢羽扇豆碱和羽扇豆碱；提取物中含量最高的化合物是金雀花碱。观察到二花卡利亚豆的粗提取物对细菌有中等活性，对植物病原真菌更有效。相比之下，金雀花碱表现出相反的效果。
