Sadeghian Hamid, Varasteh Naiemeh, Esmaeelzadeh Abbas, Nomani Hosein, Alimardani Maliheh, Davoodnejad Mahdieh, Meshkat Mojtaba, Ahadi Mitra, Sepahi Samaneh, Rostami Sina, Meshkat Zahra
Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, IR Iran.
School of Medicine, Islamic Azad University, Mashhad Branch, Mashhad, IR Iran.
Jundishapur J Microbiol. 2015 Feb 20;8(2):e14908. doi: 10.5812/jjm.14908. eCollection 2015 Feb.
Hepatitis delta virus (HDV) is dependent on the hepatitis B virus for transmission and propagation. Based on isolated HDV sequences from different parts of the world, at least three major different genotypes with different geographic distributions are suggested. Studies have shown that genotype 1 is the predominant genotype of HDV in different parts of Iran; however, the genotype distribution of this virus has not been identified in Mashhad, northeast Iran.
This current study determines the frequency of HDV major genotypes in Mashhad, Iran.
Twenty-five participants were enrolled in this study. All samples were positive for HBsAg (determined by Enzyme-linked immunosorbent assay (ELISA)) and anti-HDV. RNA extraction and cDNA synthesis was performed. Then, PCR was performed and HDV genotypes were determined by restriction fragment length polymorphism (RFLP).
Of 25 patients, 12 (48%) were positive for HDV RNA. Genotype analysis of HDV RNA revealed that the prevalence of HDV genotypes I and II was 83.3% (n = 10) and 16.7% (n = 2), respectively.
This study showed that the most prevalent genotype of HDV in Mashhad was genotype I. It was of interest that in contrast to other provinces of Iran, HDV genotype 2 was observed in Mashhad. Similar studies with larger sample sizes could provide valuable information regarding the molecular epidemiology and geographical distribution. It may also help control and prevent the spread of hepatitis D virus infections. In addition, the genotyping of HDV may predict the severity of the disease.
丁型肝炎病毒（HDV）依赖乙型肝炎病毒进行传播和扩散。基于从世界各地分离出的HDV序列，提示至少存在三种具有不同地理分布的主要不同基因型。研究表明，基因型1是伊朗不同地区HDV的主要基因型；然而，在伊朗东北部的马什哈德，尚未确定该病毒的基因型分布情况。
本研究旨在确定伊朗马什哈德HDV主要基因型的频率。
本研究纳入了25名参与者。所有样本的乙肝表面抗原（通过酶联免疫吸附测定（ELISA）确定）和抗HDV均呈阳性。进行了RNA提取和cDNA合成。然后，进行PCR，并通过限制性片段长度多态性（RFLP）确定HDV基因型。
25例患者中，12例（48%）HDV RNA呈阳性。HDV RNA的基因型分析显示，HDV基因型I和II的流行率分别为83.3%（n = 10）和16.7%（n = 2）。
本研究表明，马什哈德HDV最常见的基因型是基因型I。有趣的是，与伊朗其他省份不同，在马什哈德观察到了HDV基因型2。样本量更大的类似研究可能会提供有关分子流行病学和地理分布的有价值信息。这也可能有助于控制和预防丁型肝炎病毒感染的传播。此外，HDV基因分型可能预测疾病的严重程度。
