Cernanská Dana, Paoletti Barbara, Králová-Hromadová Ivica, Iorio Raffaella, Cudeková Patrícia, Milillo Piermarino, Traversa Donato
Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001 Kosice, Slovak Republic.
Vet Parasitol. 2009 Mar 9;160(1-2):171-4. doi: 10.1016/j.vetpar.2008.10.078. Epub 2008 Nov 1.
Five horse farms located in eastern Slovakia were investigated for the presence of benzimidazole-resistant strongyles by faecal egg count reduction test and egg hatch assay. Coprocultures were prepared for each farm from faecal samples taken pre- and post-treatment and harvested larvae were molecularly examined with a Reverse Line Blot assay. Faecal egg count reduction values ranged from 0 to 52.5% and all farms were positive for benzimidazole-resistant cyathostomins. Seven benzimidazole-resistant cyathostomin species were molecularly identified on farms before and also after treatment. These data demonstrate that resistance to benzimidazoles is well established in cyathostomin populations from horse farms in the Slovak Republic and that the molecular assay was able to determine the species-specific distribution of resistant cyathostomins under field conditions.
通过粪便虫卵计数减少试验和虫卵孵化试验，对位于斯洛伐克东部的五个马场进行了抗苯并咪唑类圆线虫的调查。从每个马场治疗前后采集的粪便样本中制备粪培养物，并对收获的幼虫进行反向线印迹分析的分子检测。粪便虫卵计数减少值范围为0至52.5%，所有马场的抗苯并咪唑类杯状类圆线虫均呈阳性。在治疗前后的马场中，分子鉴定出了七种抗苯并咪唑类杯状类圆线虫。这些数据表明，斯洛伐克共和国马场的杯状类圆线虫种群对苯并咪唑类药物具有良好的抗性，并且分子检测能够在田间条件下确定抗杯状类圆线虫的物种特异性分布。
