Faculty of Veterinary Medicine, Department of Comparative Biomedical Sciences, University of Teramo, Italy.
Vet Parasitol. 2010 Feb 26;168(1-2):84-92. doi: 10.1016/j.vetpar.2009.10.006. Epub 2009 Nov 10.
A broad scale study was carried out in 2008 to evaluate the distribution and species-specific occurrence of cyathostomin populations in horse yards from Europe. In total 102 properties and 3123 horses were included in Italy (60 yards and 1646 animals), United Kingdom (22 yards and 737 animals) and Germany (20 yards and 740 animals). Individual faecal samples were examined with a McMaster technique while pooled samples were subjected to the microscopic examination of in vitro cultured larvae and to a Reverse Line Blot (RLB) assay able to molecularly identify the most diffused 13 species of cyathostomins. All yards were positive for the presence of cyathostomins both at the McMaster technique and at the microscopic examination of cultured larvae. One thousand and nine hundred thirty-one horses (61.8%) showed a positive faecal egg count, i.e. 1110 (67.4%), 463 (62.8%) and 358 (48.3%) from Italy, UK and Germany respectively. Out of the 1931 positive animals 1133 (36.3%) showed a faecal egg count per gram >150, specifically 694 (42.2%) from Italy, 237 (32.2%) from UK and 202 (27.3%) from Germany. The molecular results showed that all 13 species that can be detected by the RLB were found in each of the three countries, with a range of 3-13 species present in individual yards. The five most prevalent were Cylicocyclus nassatus, Cylicostephanus longibursatus, Cyathostomum catinatum, Cylicocyclus goldi and Cyathostomum pateratum. The relevance of these results and related biological and epidemiological features are discussed, together with their significance for both future studies of cyathostomins and further intervention programs aiming to control the spread of anthelmintic-resistant populations.
2008 年进行了一项广泛的研究，以评估欧洲马厩中旋毛虫种群的分布和物种特异性。共有 102 个物业和 3123 匹马被纳入意大利（60 个物业和 1646 匹马）、英国（22 个物业和 737 匹马）和德国（20 个物业和 740 匹马）。个体粪便样本用麦克马斯特技术检查，而混合样本则进行体外培养幼虫的显微镜检查和反转线点杂交（RLB）分析，以分子鉴定最广泛传播的 13 种旋毛虫。所有马厩在麦克马斯特技术和培养幼虫的显微镜检查中均检测到旋毛虫存在。1093 匹马（61.8%）粪便卵计数阳性，即意大利 1110 匹马（67.4%）、英国 463 匹马（62.8%）和德国 358 匹马（48.3%）。在 1931 头阳性动物中，1133 头（36.3%）粪便卵计数每克>150，具体而言，意大利 694 头（42.2%）、英国 237 头（32.2%）和德国 202 头（27.3%）。分子结果表明，RLB 可检测到的 13 种旋毛虫在这三个国家的每一个国家均存在，每个马厩中存在 3-13 种旋毛虫。最常见的是 Cylicocyclus nassatus、Cylicostephanus longibursatus、Cyathostomum catinatum、Cylicocyclus goldi 和 Cyathostomum pateratum。讨论了这些结果及其相关生物学和流行病学特征的相关性，以及它们对未来旋毛虫研究和旨在控制驱虫剂耐药种群传播的进一步干预计划的意义。
