Vigliano F A, Bermúdez R, Nieto J M, Quiroga M I
Cátedra de Histología y Embriología, Facultad de Ciencias Veterinarias, Universidad Nacional de Rosario, Casilda, Argentina.
Fish Shellfish Immunol. 2009 Jan;26(1):146-53. doi: 10.1016/j.fsi.2008.02.016. Epub 2008 Mar 5.
Rodlet cells are an enigmatic cell type described in tissues of both marine and freshwater teleosts. Although their structure is well established, up to date their function remains subject of debate. However, there is consensus among the majority of researchers that rodlet cells play an important role within immune system, and this function is probably related with the release of rodlets due to contractile capability of their fibrous layer. Regulation of the contraction mechanism would require proteins that modulate Ca(++) intracellular concentration to be expressed in rodlet cells. We performed a morphological and immunohistochemical study at light and electron microscopy levels to assess S100 protein immunoreactivity in developing rodlet cells. Immature stages did not exhibit immunoreactive signal; however, immunoreactivity was observed in the fibrous layer of both transitional and mature rodlet cells. The latter stage also showed immunosignal within the rodlets. These findings suggest a clear association between S100 protein expression and rodlet cell development that could be linked to the regulation of rodlet activity and contractile property of their fibrous layer. Furthermore, S100 protein antibody constitutes a novel marker for rodlet cells that could be used in future studies of this particular cell type.
小杆细胞是一种神秘的细胞类型，在海洋和淡水硬骨鱼的组织中均有描述。尽管其结构已明确，但迄今为止其功能仍存在争议。然而，大多数研究人员达成的共识是，小杆细胞在免疫系统中发挥重要作用，并且这种功能可能与由于其纤维层的收缩能力而释放小杆有关。收缩机制的调节将需要在小杆细胞中表达调节细胞内钙离子浓度的蛋白质。我们在光学和电子显微镜水平上进行了形态学和免疫组织化学研究，以评估发育中小杆细胞中的S100蛋白免疫反应性。未成熟阶段未显示免疫反应信号；然而，在过渡型和成熟型小杆细胞的纤维层中均观察到免疫反应性。后期在小杆内也显示出免疫信号。这些发现表明S100蛋白表达与小杆细胞发育之间存在明显关联，这可能与小杆活性及其纤维层收缩特性的调节有关。此外，S100蛋白抗体构成了一种用于小杆细胞的新型标记物，可用于未来对这种特殊细胞类型的研究。
