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宾尼鱼肠道中棒状细胞的免疫功能（）。

The immunity function of rodlet cells in the intestine of Binni fish ().

作者信息

Karim Abdulkarim Jafar, Hassan Ali Hussein, Kadhim Khalid Hadi, Kadhim Khalid Kamil

机构信息

Department of Anatomy, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.

College of Dentistry, University of Sulaimani, Sulaymaniyah, Iraq.

出版信息

J Adv Vet Anim Res. 2022 Jun 27;9(2):282-289. doi: 10.5455/javar.2022.i594. eCollection 2022 Jun.

DOI:10.5455/javar.2022.i594
PMID:35891664
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9298095/
Abstract

OBJECTIVE

Rodlet cells produce secretions of glycoproteins in nature. This study investigated the microscopic morphology, histochemical and immunohistochemical reactions, and distribution of the rodlet cells in the gut of Binni fish (Mesopotamichthys sharpeyi).

MATERIALS AND METHODS

Thirty samples were obtained from the cranial, middle, and caudal portions of Binni intestine immediately after being euthanized, fixed in Bouin's solution for 18 h at 24°C, and had undergone routine histological processing, different conventional histochemical stains, and immunostaining with TNF-α and S100 protein antibody.

RESULTS

The intestine of Binni fish showed different stages of rodlet cells classified into three distinctive forms: vesicular, granular, and mature cells. Rodlet cells are poorly stained with hematoxylin and eosin. Their secretory granules have a weak positive reaction with periodic acid-Schiff (PAS) and Alcian blue (AB), and react positively to combined AB and PAS. Rodlet cells were stained lightly with Safranin O, observed pink in color by Giemsa stain, and showed reactivity to Masson's and Mallory trichrome stains. Rodlet cells were immunostained positively against TNF-α and S100 antibodies, indicating that they have an immune function.

CONCLUSIONS

Rodlet cells, with their neutral glycoprotein secretions, play a crucial role in the immunity of Binni fish intestine.

摘要

目的

杆状细胞在自然界中产生糖蛋白分泌物。本研究调查了宾尼鱼（Mesopotamichthys sharpeyi）肠道中杆状细胞的微观形态、组织化学和免疫组织化学反应以及分布情况。

材料与方法

在30条宾尼鱼安乐死后，立即从其肠道的头、中、尾部分获取样本，于24°C下用Bouin氏液固定18小时，然后进行常规组织学处理、不同的传统组织化学染色以及用肿瘤坏死因子-α（TNF-α）和S100蛋白抗体进行免疫染色。

结果

宾尼鱼的肠道显示出杆状细胞的不同阶段，分为三种不同形态：泡状、颗粒状和成熟细胞。杆状细胞苏木精-伊红染色不佳。其分泌颗粒对过碘酸-希夫（PAS）和阿尔辛蓝（AB）呈弱阳性反应，对AB和PAS联合染色呈阳性反应。杆状细胞番红O染色浅，吉姆萨染色呈粉红色，对Masson三色染色和马洛里三色染色有反应。杆状细胞对TNF-α和S100抗体免疫染色呈阳性，表明它们具有免疫功能。

结论

杆状细胞及其分泌的中性糖蛋白在宾尼鱼肠道免疫中起关键作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/3efed6a9d630/JAVAR-9-282-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/fc84771ae976/JAVAR-9-282-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/c65f893fe163/JAVAR-9-282-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/8c66b2453405/JAVAR-9-282-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/5dc47e73dc63/JAVAR-9-282-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/54baa2c8e3ea/JAVAR-9-282-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/f91dffec1ba7/JAVAR-9-282-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/c75fb1d2b2a5/JAVAR-9-282-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/3efed6a9d630/JAVAR-9-282-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/fc84771ae976/JAVAR-9-282-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/c65f893fe163/JAVAR-9-282-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/8c66b2453405/JAVAR-9-282-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/430d174a2587/JAVAR-9-282-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/5dc47e73dc63/JAVAR-9-282-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/54baa2c8e3ea/JAVAR-9-282-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/f91dffec1ba7/JAVAR-9-282-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/c75fb1d2b2a5/JAVAR-9-282-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a040/9298095/3efed6a9d630/JAVAR-9-282-g009.jpg

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