Harmasheva I L, Kovalenko N K
Mikrobiol Z. 2008 Jul-Aug;70(4):31-9.
The strains with antagonistic activity against indicator strains Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumonia, Salmonella enterica were revealed among 58 Enterococcus strains isolated from gastrointestinal tract of long-livers. The influence of different components of MRS medium on the antagonistic effect was established. Antagonistic activity remained on the medium without glucose in 36% of strains, on the medium without tween 80--in 60% of strains, on the medium without sodium acetate and ammonium citric acid--in 65% of strains. The activity both for the lack of K2HPO4, MgSO4 and MnSO4, and in the absence of complex nitrogen sources (peptone, beef extract, yeast extract and hepatic extract) was shown in 76% of strains.
从长寿者胃肠道分离出的58株肠球菌中,发现了对指示菌株铜绿假单胞菌、普通变形杆菌、金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、肺炎克雷伯菌、肠炎沙门氏菌具有拮抗活性的菌株。确定了MRS培养基不同成分对拮抗作用的影响。36%的菌株在不含葡萄糖的培养基上仍具有拮抗活性,60%的菌株在不含吐温80的培养基上具有拮抗活性,65%的菌株在不含醋酸钠和柠檬酸铵的培养基上具有拮抗活性。76%的菌株在缺乏K2HPO4、MgSO4和MnSO4以及在没有复合氮源(蛋白胨、牛肉浸膏、酵母浸膏和肝脏浸膏)的情况下均表现出活性。