通过径向流亲和色谱法对纤溶酶原进行亲和纯化。
Affinity purification of plasminogen by radial-flow affinity chromatography.
作者信息
Planques Y, Pora H, Menozzi F D
机构信息
Institut Pasteur, Laboratoire de Microbiologie Moléculaire, Lille, France.
出版信息
J Chromatogr. 1991 Feb 22;539(2):531-3. doi: 10.1016/s0021-9673(01)83965-0.
PMID:1904448
Abstract
A method for the purification of plasminogen using immobilized L-lysine on a membrane, the whole system being constructed in a radial flow cartridge, is described. Human plasma was applied to the cartridge at 20 ml/min. The results showed that under the chromatographic conditions chosen, in a single pass, greater than 85% recovery of plasminogen was attained with a 110-fold increase in specific activity.
摘要
描述了一种使用固定在膜上的L-赖氨酸在径向流柱中构建的整个系统纯化纤溶酶原的方法。以20毫升/分钟的流速将人血浆施加到柱中。结果表明，在所选择的色谱条件下，单次通过时，纤溶酶原的回收率大于85%，比活性提高了110倍。
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