Kudinov S A, Eretskaia E V, Pozdnikova T M, Panchenko N E, Matsyĭ S P
Ukr Biokhim Zh (1978). 1979 Jul-Aug;51(4):330-4.
Using immobilization of l-lysine on cellulose and CM-Cellulose activated with bromo-cyanogen and dicyclohexyl carboxyimide, respectively, the affined sorbents are obtained and possibility of their application for obtaining the high-purified plasminogen is studied. According to the yield and activity of plasminogen, l-lysine-cellulose and l-lysine-CM-cellulose are not inferior to the known l-lysine-sepharose. With electrophoresis in polyacrylamide gel in the presence of sodium dodecyl sulphate the plasminogen preparations obtained by the method of affined chromatography are homogeneous and their molecular mass is 87 000 Daltons.
分别通过将L-赖氨酸固定在经溴化氰和二环己基碳二亚胺活化的纤维素和羧甲基纤维素上,获得了亲和吸附剂,并研究了其用于获得高纯度纤溶酶原的可能性。根据纤溶酶原的产率和活性,L-赖氨酸-纤维素和L-赖氨酸-羧甲基纤维素并不逊色于已知的L-赖氨酸-琼脂糖。在十二烷基硫酸钠存在下进行聚丙烯酰胺凝胶电泳时,通过亲和色谱法获得的纤溶酶原制剂是均一的,其分子量为87000道尔顿。