Comps M, Mari J, Poisson F, Bonami J R
GIE.RA-Station Expérimentale d'Aquaculture de l'Ifremer, Palavas-les-Flots, France.
J Gen Virol. 1991 Jun;72 ( Pt 6):1229-36. doi: 10.1099/0022-1317-72-6-1229.
A cytoplasmic dsRNA virus, rotifer birnavirus (RBV), has recently been isolated from the rotifer Brachionus plicatilis and is associated with a high mortality rate. Histologically, the viral lesions consist of characteristic inclusions, particularly amorphous dense bodies containing occluded particles. Purified virions are about 59 nm in diameter, single-shelled and display four capsomers per edge. The purified virions have a buoyant density of 1.290 (full particles) and 1.250 (empty particles) in CsCl gradients. Four major structural polypeptides of MrS 60K, 52K, 33K and 27K were detected by SDS-PAGE. The genome is composed of two linear segments of dsRNA with MrS of 2.45 x 10(6) and 2.31 x 10(6); additionally, small circular ssRNA molecules were detected by electrophoresis in overloaded agarose gels, but their significance is currently unknown. Except for this last feature and the structural instability of purified virions under freeze storage, all the other biochemical and biophysical characters indicate that RBV is a member of the Birnaviridae family with, for the moment, a unique position in this group.
一种细胞质双链RNA病毒，即轮虫双RNA病毒（RBV），最近从褶皱臂尾轮虫中分离出来，并且与高死亡率有关。在组织学上，病毒损伤由特征性内含物组成，特别是含有包埋颗粒的无定形致密体。纯化的病毒粒子直径约59纳米，为单壳，每条边显示四个壳粒。在氯化铯梯度中，纯化的病毒粒子的浮力密度为1.290（完整粒子）和1.250（空粒子）。通过SDS-PAGE检测到分子量分别为60K、52K、33K和27K的四种主要结构多肽。基因组由两条双链RNA线性片段组成，分子量分别为2.45×10⁶和2.31×10⁶；此外，在过载琼脂糖凝胶电泳中检测到小的环状单链RNA分子，但其意义目前尚不清楚。除了这最后一个特征以及纯化病毒粒子在冷冻保存下的结构不稳定性外，所有其他生化和生物物理特征表明RBV是双RNA病毒科的成员，目前在该类群中处于独特地位。
