Winton J R, Lannan C N, Fryer J L, Hedrick R P, Meyers T R, Plumb J A, Yamamoto T
J Gen Virol. 1987 Feb;68 ( Pt 2):353-64. doi: 10.1099/0022-1317-68-2-353.
The morphological, biochemical and growth characteristics of four members of the Reoviridae, three from the fish hosts, golden shiner (Notemigonus crysoleucas), chum salmon (Oncorhynchus keta) and channel catfish (Ictalurus punctatus) and one from American oyster (Crassostrea virginica), were compared. Electron microscopy of negatively stained virions revealed icosahedral particles approximately 75 nm in diameter composed of a double capsid. Complete particles had buoyant densities in CsCl of 1.34 to 1.36 g/ml. The viruses replicated well in several fish cell lines, forming plaque-like syncytia in monolayer cultures. Each virus could be distinguished by the range of cell lines supporting its growth. Polyacrylamide gel electrophoresis showed that the genome of each virus was composed of 11 segments of dsRNA distributed among three size classes. There were three large, three medium and five small segments in each genome and each isolate had a unique electropherotype. The segments ranged from 2.5 X 10(6) to 0.31 X 10(6) mol. wt. with a total genome of approximately 15 X 10(6) mol. wt. Analysis by SDS-PAGE revealed that each virus had five major structural proteins. There were two large polypeptides of approximately 135,000 and 125,000 mol. wt., one medium size polypeptide of 70,000 mol. wt. and two small polypeptides of 45,000 and 34,000 mol. wt. Of the major structural proteins, those of approximately 70,000 and 34,000 mol. wt. were consistently present in the highest concentrations. Minor virion proteins were detected but were not characterized. These four viruses, isolated from aquatic animals, were unlike viruses of the six established genera of the Reoviridae.
比较了呼肠孤病毒科4个成员的形态、生化和生长特性，其中3个分离自鱼类宿主，即美国拟鲤（Notemigonus crysoleucas）、大麻哈鱼（Oncorhynchus keta）和斑点叉尾鮰（Ictalurus punctatus），1个分离自美洲牡蛎（Crassostrea virginica）。对经负染的病毒粒子进行电子显微镜观察，发现直径约75 nm的二十面体粒子由双层衣壳组成。完整粒子在CsCl中的浮力密度为1.34至1.36 g/ml。这些病毒在几种鱼类细胞系中复制良好，在单层培养物中形成斑块状多核体。每种病毒可通过支持其生长的细胞系范围加以区分。聚丙烯酰胺凝胶电泳显示，每种病毒的基因组由11条双链RNA片段组成，分布在3个大小类别中。每个基因组中有3条大片段、3条中片段和5条小片段，每个分离株都有独特的电泳图谱型。这些片段的分子量范围为2.5×10⁶至0.31×10⁶，基因组总分子量约为15×10⁶。SDS-PAGE分析表明，每种病毒都有5种主要结构蛋白。有两种大约135,000和125,000分子量的大分子量多肽、一种70,000分子量的中分子量多肽以及两种45,000和34,000分子量的小分子量多肽。在主要结构蛋白中，分子量约为70,000和34,000的蛋白始终以最高浓度存在。检测到了次要的病毒粒子蛋白，但未对其进行鉴定。这4种从水生动物中分离出的病毒与呼肠孤病毒科6个已确立属的病毒不同。
