Todd D, Creelan J L, Mackie D P, Rixon F, McNulty M S
Virology Section, Veterinary Research Laboratories, Stormont, Belfast, U.K.
J Gen Virol. 1990 Apr;71 ( Pt 4):819-23. doi: 10.1099/0022-1317-71-4-819.
Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and CsCl. The purification method was dependent on the use of an antigen-detecting ELISA based on a CAA-specific monoclonal antibody. Virus particles banded at a density of 1.33 to 1.34 g/ml in CsCl and measured 23.5 +/- 0.8 nm in diameter. Purified preparations contained one major polypeptide (Mr 50,000) and a single-stranded, circular DNA (2.3 kb). CAA shares some of the biochemical characteristics possessed by porcine circovirus and the virus associated with psittacine beak and feather disease.
利用差速离心以及使用蔗糖和氯化铯进行连续的平衡密度梯度离心循环对鸡贫血因子（CAA）进行了纯化。该纯化方法依赖于使用基于CAA特异性单克隆抗体的抗原检测酶联免疫吸附测定（ELISA）。病毒颗粒在氯化铯中的密度为1.33至1.34 g/ml，直径为23.5±0.8 nm。纯化制剂包含一种主要多肽（分子量50,000）和一条单链环状DNA（2.3 kb）。CAA具有猪圆环病毒以及与鹦鹉喙羽病相关病毒所拥有的一些生化特性。
