Hofmann Martin A, Renzullo Sandra, Mader Markus, Chaignat Valérie, Worwa Gabriella, Thuer Barbara
Institute of Virology and Immunoprophylaxis, Mittelhaeusern, Switzerland.
Emerg Infect Dis. 2008 Dec;14(12):1855-61. doi: 10.3201/eid1412.080818.
A novel bluetongue virus (BTV) termed Toggenburg orbivirus (TOV) was detected in goats from Switzerland by using real-time reverse transcription-PCR. cDNA corresponding to the complete sequence of 7 of 10 double-stranded RNA segments of the viral genome was amplified by PCR and cloned into a plasmid vector. Five clones for each genome segment were sequenced to determine a consensus sequence. BLAST analysis and dendrogram construction showed that TOV is closely related to BTV, although some genome segments are distinct from the 24 known BTV serotypes. Maximal sequence identity to any BTV ranged from 63% (segment 2) to 79% (segments 7 and 10). Because the gene encoding outer capsid protein 2 (VP2), which determines the serotype of BTV, is placed within the BTV serogroup, we propose that TOV represents an unknown 25th serotype of BTV.
通过实时逆转录聚合酶链反应（RT-PCR），在瑞士山羊中检测到一种新型蓝舌病毒（BTV），称为托根堡环状病毒（TOV）。通过PCR扩增与病毒基因组10个双链RNA片段中的7个完整序列相对应的cDNA，并将其克隆到质粒载体中。对每个基因组片段的5个克隆进行测序以确定共有序列。BLAST分析和系统发育树构建表明，TOV与BTV密切相关，尽管一些基因组片段与24种已知的BTV血清型不同。与任何BTV的最大序列同一性范围为63%（第2片段）至79%（第7和10片段）。由于决定BTV血清型的编码外衣壳蛋白2（VP2）的基因位于BTV血清群内，我们提出TOV代表BTV的一种未知的第25种血清型。
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