Savini Giovanni, Puggioni Giantonella, Meloni Giorgio, Marcacci Maurilia, Di Domenico Marco, Rocchigiani Angela Maria, Spedicato Massimo, Oggiano Annalisa, Manunta Daniela, Teodori Liana, Leone Alessandra, Portanti Ottavio, Cito Francesca, Conte Annamaria, Orsini Massimiliano, Cammà Cesare, Calistri Paolo, Giovannini Armando, Lorusso Alessio
OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale dell'Abruzzo e Molise (IZSAM), Teramo, Italy.
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.
Infect Genet Evol. 2017 Jul;51:108-117. doi: 10.1016/j.meegid.2017.03.021. Epub 2017 Mar 21.
In recent years, novel Bluetongue virus (BTV) serotypes have been isolated and/or sequenced by researchers within the field. During Bluetongue surveillance activities, we identified a putative novel BTV serotype in healthy goats from Sardinia, Italy. RNAs purified from blood and serum samples were positive for BTV by a generic real time RT-PCR and c-ELISA, respectively, whereas genotyping and serotyping were unsuccessful. By NGS, the whole genome sequence was obtained from two blood samples (BTV-X ITL2015 strains 34200 and 33531). Overall, Seg 2 of BTV-X ITL2015 shows the highest identity (75.3-75.5% nt/77.4-78.1% aa) with recently isolated BTV-27s from Corsica and with the last discovered BTV XJ1407 from China (75.9% nt /78.2% aa), whereas it is less related with BTV-25 from Switzerland (73.0% nt/75.0% aa) and BTV-26 from Kuwait (62.0% nt/60.5% aa). A specific RT-qPCR targeting Seg 2 of BTV-X ITL2015 was assessed in this study. Considering the Seg 2/VP2 identity of BTV-X ITL2015 with BTV-25, 26, 27s and BTV XJ1407 and that serum of BTV-X ITL2015 infected goats failed to neutralize all tested extant serotypes, we propose the existence of a novel BTV serotype circulating in goats in Sardinia. Isolation was so far unsuccessful thus hampering proper antigenic characterization.
近年来，该领域的研究人员分离出了新型蓝舌病毒（BTV）血清型并/或对其进行了测序。在蓝舌病监测活动中，我们在意大利撒丁岛的健康山羊中发现了一种推定的新型BTV血清型。从血液和血清样本中纯化的RNA，通过通用实时RT-PCR和c-ELISA分别检测出BTV呈阳性，而基因分型和血清分型均未成功。通过二代测序（NGS），从两份血液样本（BTV-X ITL2015毒株34200和33531）中获得了全基因组序列。总体而言，BTV-X ITL2015的第2节段与最近从科西嘉岛分离出的BTV-27以及最后发现的来自中国的BTV XJ1407显示出最高的同一性（75.3 - 75.5%核苷酸/77.4 - 78.1%氨基酸），而与来自瑞士的BTV-25（73.0%核苷酸/75.0%氨基酸）和来自科威特的BTV-26（62.0%核苷酸/60.5%氨基酸）的相关性较低。本研究评估了针对BTV-X ITL2015第2节段的特异性RT-qPCR。考虑到BTV-X ITL2015的第2节段/VP2与BTV-25、26、27以及BTV XJ1407的同一性，并且感染BTV-X ITL2015的山羊血清未能中和所有测试的现有血清型，我们提出在撒丁岛的山羊中存在一种新型BTV血清型正在传播。到目前为止，分离尚未成功，因此妨碍了进行适当的抗原特性鉴定。
