Carbonic anhydrase II in rat acid secreting cells: comparison of osteoclasts with gastric parietal cells and kidney intercalated cells.

Location of carbonic anhydrase II, an important enzyme involved in acid production, was studied using an immunogold method on ultracryosections. Its distribution in osteoclasts was compared with that in gastric parietal cells and kidney intercalated cells of the inner stripe of outer medulla. It is shown that the distribution of carbonic anhydrase II is much similar in all of these acid producing cells: most of the enzyme is cytoplasmic and nucleoplasmic and only a small fraction of the enzyme is associated with the apical plasma membrane. It seems likely that carbonic anhydrase II has a similar role in all of these acid producing cells.