Núñez P E, Scoging A C
Food Hygiene Laboratory, PHLS Central Public Health Laboratory, Colindale, London, UK.
Int J Food Microbiol. 1997 Apr 29;36(1):39-48. doi: 10.1016/s0168-1605(97)01240-3.
Consumption of shellfish contaminated with algal toxins produced by marine dinoflagellates can lead to diarrhetic shellfish poisoning (DSP). UK legislation necessitates toxin detection by mouse bioassay but this method is non-specific and lacks sensitivity. As an alternative method, an HPLC technique has been optimized, with detection limits of 0.26 micrograms of toxin/g of shellfish hepatopancreas for both Okadaic Acid (OA) and Dinophysistoxin-1 (DTX-1). A colorimetric protein phosphatase inhibition (PPI) assay has also been optimized. This assay detects inhibition of protein phosphatase 1 (PPI gamma) by OA and DTX-1 with detection limits of 1.5 ng of total toxin/g of hepatopancreas. Contaminated shellfish from several European sources, the UK monitoring programmes and mussels associated with an outbreak of DSP poisoning in the UK, have been analyzed and assessed using the two alternative methods and a commercially available enzyme-linked immunosorbent assay (ELISA) kit. The results indicate that both the HPLC and PPI assays correlate well with each other and with the UK standard mouse bioassay. In contrast, and not withstanding its advantages of rapidity and ease, the ELISA kit did not accurately and consistently detect low toxin concentrations, although it may be useful as a screening tool.
食用被海洋双鞭甲藻产生的藻类毒素污染的贝类会导致腹泻性贝类中毒（DSP）。英国立法规定通过小鼠生物测定法进行毒素检测，但该方法不具有特异性且缺乏灵敏度。作为一种替代方法，一种高效液相色谱（HPLC）技术已得到优化，对于冈田酸（OA）和鳍藻毒素-1（DTX-1），检测限均为0.26微克毒素/克贝类肝胰腺。一种比色蛋白磷酸酶抑制（PPI）测定法也已得到优化。该测定法可检测OA和DTX-1对蛋白磷酸酶1（PPIγ）的抑制作用，检测限为1.5纳克总毒素/克肝胰腺。已使用这两种替代方法以及一种市售的酶联免疫吸附测定（ELISA）试剂盒对来自欧洲多个来源的受污染贝类、英国监测计划中的贝类以及与英国一起DSP中毒事件相关的贻贝进行了分析和评估。结果表明，HPLC测定法和PPI测定法相互之间以及与英国标准小鼠生物测定法都具有良好的相关性。相比之下，尽管ELISA试剂盒具有快速简便的优点，但它不能准确且一致地检测低毒素浓度，不过它可能作为一种筛查工具是有用的。
