澳大利亚布里斯班红肉和牛奶样本中气单胞菌属和李斯特菌属的发生率。

Incidence of Aeromonas and Listeria spp. in red meat and milk samples in Brisbane, Australia.

作者信息

Ibrahim A, Mac Rae I C

机构信息

Department of Microbiology, University of Queensland, Australia.

出版信息

Int J Food Microbiol. 1991 Feb;12(2-3):263-9. doi: 10.1016/0168-1605(91)90077-3.

DOI:10.1016/0168-1605(91)90077-3

PMID:1904764

Abstract

A total of 150 samples, 50 each of beef, lamb and pork from 10 local retail stores in Brisbane metropolitan area as well as 150 raw bovine bulk milk tank samples obtained from Queensland United Foods (QUF), were examined for the presence of Aeromonas and Listeria spp. over a period of 1 year. Different sets of enrichment and plating media were used to recover the organisms with subsequent identification using conventional biochemical and serotyping techniques. A total of 509 isolates consisting of Aeromonas spp. (350) and Listeria spp. (159) were obtained from 60%, 58%, 74%, 26.6%, 34%, 40%, 30%, 2.6% of samples of beef, Lamb, pork and milk respectively. Motile aeromonads (A. hydrophila, A. sobria, A. caviae) and Listeria innocua were isolated from all kinds of samples whereas L. monocytogenes was only isolated from flesh food. A. hydrophila contributed the largest percentage of the motile aeromonads (60%). The majority of L. monocytogenes cultures were serotype 4.

摘要

在1年的时间里，对来自布里斯班都会区10家当地零售店的总共150份样本（牛肉、羊肉和猪肉各50份）以及从昆士兰联合食品公司（QUF）获取的150份生牛散装奶罐样本进行了气单胞菌属和李斯特菌属检测。使用不同组的增菌和接种培养基来分离这些微生物，随后采用传统生化和血清分型技术进行鉴定。从牛肉、羊肉、猪肉和牛奶样本中分别有60%、58%、74%、26.6%、34%、40%、30%、2.6%的样本中总共获得了509株分离株，其中气单胞菌属（350株）和李斯特菌属（159株）。从各类样本中均分离出了运动性气单胞菌（嗜水气单胞菌、温和气单胞菌、豚鼠气单胞菌）和无害李斯特菌，而单核细胞增生李斯特菌仅从肉类食品中分离得到。嗜水气单胞菌在运动性气单胞菌中所占比例最大（60%）。大多数单核细胞增生李斯特菌培养物为血清型4。