Majeed K, Egan A, MacRae I C
Department of Microbiology, University of Queensland, St Lucia, Australia.
J Appl Bacteriol. 1989 Aug;67(2):165-70. doi: 10.1111/j.1365-2672.1989.tb03391.x.
Enrichment in alkaline peptone water was compared with the direct plating method for the isolation of Aeromonas spp. from lamb meat and offal samples. The enrichment method significantly increased the isolation rate of aeromonads. Motile Aeromonas species (A. hydrophila, A. sobria and A. caviae) were present in all kinds of samples investigated. Seventy-three Aeromonas strains isolated in this survey were characterized to species level and examined for their ability to produce virulence factors. Strains identified as A. sobria were the strongest producers of haemolysin and enterotoxin, whereas A. caviae strains were consistently non-haemolytic and non-enterotoxigenic. Thus it is likely that lamb meat and offal are potentially significant sources of virulent Aeromonas species and may play an important role in the aetiology of Aeromonas-associated gastro-enteritis.
将碱性蛋白胨水增菌法与直接平板接种法用于从羊肉和内脏样本中分离气单胞菌属进行了比较。增菌法显著提高了气单胞菌的分离率。在所调查的各类样本中均存在运动性气单胞菌(嗜水气单胞菌、温和气单胞菌和豚鼠气单胞菌)。本次调查分离出的73株气单胞菌进行了种水平鉴定,并检测了它们产生毒力因子的能力。鉴定为温和气单胞菌的菌株是溶血素和肠毒素的最强生产者,而豚鼠气单胞菌菌株始终不溶血且不产肠毒素。因此,羊肉和内脏很可能是有毒力气单胞菌属的潜在重要来源,并且可能在气单胞菌相关肠胃炎的病因学中发挥重要作用。