DNA模板化金纳米颗粒的形成。

DNA-templated gold nanoparticles formation.

作者信息

Sun Lanlan, Song Yonghai, Wang Li, Sun Yujing, Guo Cunlan, Liu Zhelin, Li Zhuang

机构信息

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, 130022, China.

出版信息

J Nanosci Nanotechnol. 2008 Sep;8(9):4415-23. doi: 10.1166/jnn.2008.270.

DOI:10.1166/jnn.2008.270

PMID:19049035

Abstract

The interaction between HAuCl4 and DNA has enabled creation of DNA-templated gold nanoparticles without formation of large nanoparticles. It was found that spheral DNA-HAuCl4 hybrid of 8.7 nm in diameter, flower-like DNA-HAuCl4 hybrid, nanoparticles chains and nanoparticles network of DNA-HAuCl4 hybrid could be obtained by varying the reaction conditions, including DNA concentration and reaction temperature. The intermediate product was investigated by shortening the reaction time of DNA and HAuCl4, and the obtained nanoparticles preserved a small DNA segment, which indicated that the reaction between DNA and HAuCl4 had a process. The addition of reduction reagent resulted in DNA-templated gold nanoparticles and nanoflowers, respectively. UV-vis absorption spectra were used to characterize the DNA-HAuCl4 hybrid and the gold nanostructures templated on DNA, and XPS spectra were used to compare the composition of DNA-Au(III) complex and gold nanoparticles. AFM and TEM results revealed that the spheral gold nanoparticles of about 11 nm in size and flower-like gold nanoparticles were formed after the addition of NaBH4.

摘要

HAuCl4与DNA之间的相互作用使得能够在不形成大尺寸纳米颗粒的情况下制备DNA模板化金纳米颗粒。研究发现，通过改变反应条件，包括DNA浓度和反应温度，可以获得直径为8.7 nm的球形DNA-HAuCl4杂化物、花状DNA-HAuCl4杂化物、纳米颗粒链以及DNA-HAuCl4杂化物的纳米颗粒网络。通过缩短DNA与HAuCl4的反应时间对中间产物进行了研究，所得纳米颗粒保留了一小段DNA片段，这表明DNA与HAuCl4之间的反应有一个过程。还原试剂的加入分别导致了DNA模板化金纳米颗粒和纳米花的形成。紫外可见吸收光谱用于表征DNA-HAuCl4杂化物以及DNA模板化的金纳米结构，X射线光电子能谱用于比较DNA-Au(III)络合物和金纳米颗粒的组成。原子力显微镜和透射电子显微镜结果显示，加入NaBH4后形成了尺寸约为11 nm的球形金纳米颗粒和花状金纳米颗粒。