Choi Jonghoon, Park Sungwook, Stojanović Zoran, Han Hyung-Seop, Lee Jongwook, Seok Hyun Kwang, Uskoković Dragan, Lee Kwan Hyi
Executive Office, Institute of Research Strategy and Development (IRSD) , Seoul 137-781, Republic of Korea.
Langmuir. 2013 Dec 17;29(50):15698-703. doi: 10.1021/la403888f. Epub 2013 Dec 5.
Herein, we report a quick and simple synthesis of water-soluble gold nanoparticles using a HAuCl4 and oleylamine mixture. Oleylamine serves as a reduction agent as well as a stabilizer for nanoparticle surfaces. The particle sizes can be adjusted by modulating reaction temperature and time. Solvothermal reduction of HAuCl4 with oleylamine can be confirmed by measuring the product in Fourier transform infrared (FTIR) spectroscopy. The plasmon band shifting from yellow to red confirms a nanosized particle formation. Amide bonds on the surface of the nanoparticles formed hydrogen bonds with one another, resulting in a hydrophobic monolayer. Particles dispersed well in nonpolar organic solvents, such as in hexane or toluene, by brief sonication. Next, we demonstrated the transfer of gold nanoparticles into water by lipid capsulation using 1-myristoyl-2-hydroxy-sn-glycero-3-phosphocholine (MHPC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy polyethylene glycol)-2000 (DPPE-PEG2k), and 1,2-dioleoyl-sn-glycero-3-N-{5-amino-1-carboxypentyl}iminodiacetic acid succinyl nickel salt [DGS-NTA(Ni)]. The particle concentration can be obtained using an absorbance in ultraviolet-visible (UV-vis) spectra (at 420 nm). Instrumental analyses using transmission electron microscopy (TEM), energy-dispersive X-ray (EDX) analysis, dynamic light scattering (DLS), and FTIR confirmed successful production of gold nanoparticles and fair solubility in water. Prepared gold particles were selectively clustered via engineered ferritin nanocages that provide multiple conjugation moieties. A total of 5-6 gold nanoparticles were clustered on a single ferritin nanocage confirmed in TEM. Reported solvothermal synthesis and preparation of gold nanoclusters may serve as an efficient, alternate way of preparing water-soluble gold nanoparticles, which can be used in a wide variety of biomedical applications.
在此，我们报道了一种使用四氯合金酸（HAuCl4）和油胺混合物快速简便地合成水溶性金纳米颗粒的方法。油胺既作为还原剂，又作为纳米颗粒表面的稳定剂。通过调节反应温度和时间可以调整颗粒大小。用傅里叶变换红外光谱（FTIR）测量产物可证实油胺对四氯合金酸的溶剂热还原。等离子体激元带从黄色变为红色证实了纳米颗粒的形成。纳米颗粒表面的酰胺键相互形成氢键，从而形成疏水单分子层。通过短暂超声处理，颗粒能很好地分散在非极性有机溶剂中，如己烷或甲苯中。接下来，我们展示了使用1 - 肉豆蔻酰 - 2 - 羟基 - sn - 甘油 - 3 - 磷酸胆碱（MHPC）、1,2 - 二硬脂酰 - sn - 甘油 - 3 - 磷酸乙醇胺 - N - （甲氧基聚乙二醇） - 2000（DPPE - PEG2k）和1,2 - 二油酰 - sn - 甘油 - 3 - N - {5 - 氨基 - 1 - 羧基戊基}亚氨基二乙酸琥珀酰镍盐[DGS - NTA（Ni）]通过脂质包封将金纳米颗粒转移到水中。颗粒浓度可以通过紫外 - 可见（UV - vis）光谱（在420nm处）的吸光度获得。使用透射电子显微镜（TEM）、能量色散X射线（EDX）分析、动态光散射（DLS）和FTIR进行的仪器分析证实了金纳米颗粒的成功制备及其在水中的良好溶解性。制备的金颗粒通过提供多个共轭部分的工程化铁蛋白纳米笼进行选择性聚集。在TEM中证实单个铁蛋白纳米笼上总共聚集了5 - 6个金纳米颗粒。报道的金纳米簇的溶剂热合成和制备方法可能是一种高效的、替代的制备水溶性金纳米颗粒的方法，可用于多种生物医学应用。
