Väisänen O M, Mwaisumo N J, Salkinoja-Salonen M S
University of Helsinki, Department of General Microbiology, Finland.
J Appl Bacteriol. 1991 Apr;70(4):315-24. doi: 10.1111/j.1365-2672.1991.tb02942.x.
A total of 130 Bacillus strains were isolated from dairy products, the dairy environment and from packaging boards and board-producing machines. Ninety-eight of these were members of the B. cereus group (B. cereus, B. mycoides and B. thuringiensis) as determined by whole cell fatty acid composition. Fatty acid composition did not differentiate between the three species. Of the 98 strains, which were indistinguishable by biochemical tests, 87 could be assigned into 21 different phage types (11 strains remained untypable) when tested with 12 B. cereus, B. mycoides and B. thuringiensis phages. The distribution of phage types between strains from different sources showed that the source of contamination of the dairy products was of milk origin and not from the packaging materials. Most strains isolated from the dairy products were able to grow below 10 degrees C, whereas strains from the dairy environment and from board mills had higher minimum growth temperatures.
共从乳制品、乳制品生产环境以及包装板和制板机器中分离出130株芽孢杆菌菌株。通过全细胞脂肪酸组成测定，其中98株属于蜡样芽孢杆菌群（蜡样芽孢杆菌、蕈状芽孢杆菌和苏云金芽孢杆菌）。脂肪酸组成无法区分这三个物种。在这98株通过生化试验无法区分的菌株中，用12种蜡样芽孢杆菌、蕈状芽孢杆菌和苏云金芽孢杆菌噬菌体进行检测时，87株可分为21种不同的噬菌体类型（11株仍无法分型）。不同来源菌株之间噬菌体类型的分布表明，乳制品的污染源是牛奶来源，而非包装材料。从乳制品中分离出的大多数菌株能够在10摄氏度以下生长，而从乳制品生产环境和制板厂分离出的菌株最低生长温度较高。
