Celebi Ozgur, Atabay H Ibrahim
Department of Microbiology, Veterinary Faculty, University of Kafkas, Kars, Turkey.
Trop Anim Health Prod. 2009 Jan;41(1):115-9. doi: 10.1007/s11250-008-9165-y. Epub 2008 Apr 20.
This study was undertaken to investigate the seroprevalence of brucellosis in unvaccinated sheep from the flocks having previous abortion cases in Kars and around, Turkey and to compare the efficacy of each serological test used. Four hundred serum samples collected from 16 different flocks of sheep having a history of abortions in Kars and its surrounding area in Turkey were examined for the presence of antibodies raised against Brucella using Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT), Rivanol Agglutination Test (RAT) and Complement Fixation Test (CFT). All animals were unvaccinated against Brucella. Of the serum samples tested, 147 (%36.7), 142 (%35.5), 139 (%34.75) and 135 (%33.75) were found positive by SAT, RAT, RBPT and CFT, respectively. No statistically significant difference was found between the serological tests used (p > 0.05). It is concluded from this study that brucellosis continues to be an important problem for ovine abortions and poses a risk both for human and other animals in this area. Therefore, adequate intervention measures should be implemented to control and eradicate brucellosis. In addition, if conventional serological tests are used at least two tests, RPBT for screening and CFT for the confirmation of the positive samples are preferable, should be used in parallel for detection of brucellosis effectively.
本研究旨在调查土耳其卡尔斯及其周边地区曾有流产病例的未接种疫苗羊群中布鲁氏菌病的血清阳性率,并比较所使用的每种血清学检测方法的效果。从土耳其卡尔斯及其周边地区16个有流产病史的不同羊群中采集了400份血清样本,使用玫瑰红平板试验(RBPT)、血清凝集试验(SAT)、利凡诺凝集试验(RAT)和补体结合试验(CFT)检测针对布鲁氏菌产生的抗体。所有动物均未接种布鲁氏菌疫苗。在所检测的血清样本中,SAT、RAT、RBPT和CFT分别检测出147份(36.7%)、142份(35.5%)、139份(34.75%)和135份(33.75%)呈阳性。所使用的血清学检测方法之间未发现统计学上的显著差异(p>0.05)。从本研究得出结论,布鲁氏菌病仍然是绵羊流产的一个重要问题,并且对该地区的人类和其他动物都构成风险。因此,应实施适当的干预措施来控制和根除布鲁氏菌病。此外,如果使用传统血清学检测方法,至少应同时使用两种检测方法,筛查时首选RPBT,阳性样本确认时首选CFT,以便有效地检测布鲁氏菌病。