Laboratory for Irrigation Water Protection, Biology Department - University of Regina, Regina, SK, Canada.
J Appl Microbiol. 2009 Feb;106(2):442-54. doi: 10.1111/j.1365-2672.2008.04012.x. Epub 2008 Nov 28.
To assess levels of faecal contamination in the Qu'Appelle River (Saskatchewan, Canada) and its suitability for irrigation, by using the Colilert-18/Quanti-Tray technology.
Various sites located along the Qu'Appelle River were sampled weekly from May to August 2005-2007. A total of 594 freshwater samples were collected and analysed for enumeration of Escherichia coli using the Colilert-18. The false-positive rate for E. coli detection using Colilert-18 was at most 1.5%. Throughout the irrigation period (June to August), up to 85% of the water samples collected from one of the irrigation water-pumping sites exceeded the recommended limit of 100 CFU per 100 ml. Spikes in E. coli counts were generally concomitant with the sudden rise in river flows. A sub-sample of confirmed E. coli isolates were typed by randomly amplified polymorphic DNA (RAPD). RAPD analysis revealed a high degree of genetic diversity among E. coli isolates. A significant association between RAPD patterns and the month of E. coli isolation was demonstrated.
Colilert-18 provides an effective means for assessing microbial quality of irrigation water.
Qu'Appelle River is subject to variability of faecal contamination during irrigation times and monitoring throughout irrigation season is important for ensuring safe production practices.
使用 Colilert-18/Quanti-Tray 技术评估加拿大萨斯喀彻温省夸珀尔河（Qu'Appelle River）的粪便污染水平及其用于灌溉的适宜性。
2005 年至 2007 年 5 月至 8 月期间，每周在夸珀尔河沿线的不同地点进行采样。共采集了 594 个淡水样本，使用 Colilert-18 对大肠杆菌进行计数。使用 Colilert-18 检测大肠杆菌的假阳性率最高为 1.5%。在整个灌溉期（6 月至 8 月），从一个灌溉水泵站点采集的水样中，多达 85%的水样超过了建议的 100 CFU/100ml 的限值。大肠杆菌计数的峰值通常与河水流量的突然增加同时出现。对经证实的大肠杆菌分离株的亚样本进行了随机扩增多态性 DNA（RAPD）分型。RAPD 分析显示大肠杆菌分离株之间具有高度的遗传多样性。RAPD 模式与大肠杆菌分离月份之间存在显著关联。
Colilert-18 为评估灌溉用水的微生物质量提供了一种有效的手段。
夸珀尔河在灌溉期间易受粪便污染的变化影响，因此在灌溉季节进行监测对于确保安全的生产实践非常重要。
