评估Colilert-18用于检测热带淡水中的大肠菌群和大肠杆菌。
Evaluation of Colilert-18 for the detection of coliforms and Escherichia coli in tropical fresh water.
作者信息
Chao W L
机构信息
Department of Microbiology, Soochow University, Shih Lin, Taipei, Taiwan.
出版信息
Lett Appl Microbiol. 2006 Feb;42(2):115-20. doi: 10.1111/j.1472-765X.2005.01814.x.
AIMS
To evaluate the suitability of Colilert-18 in detecting Escherichia coli and total coliforms in tropical freshwater samples.
METHODS AND RESULTS
Target organisms were isolated from yellow-fluorescent and yellow wells of Colilert-18/Quanti-Tray using m-TEC agar and m-ENDO LES agar respectively. All the selected isolates were first identified based on their fatty acid methyl ester profile. Isolates showing contradictory results to that of the Colilert-18 procedure were re-identified using API 20E strips. A total of 357 isolates, 177 from yellow-fluorescent wells and 180 from yellow wells, were identified.
CONCLUSIONS
The false-positive and -negative rates for E. coli detection using Colilert-18 were 36.4% and 11%, respectively, while for coliform detection the false-positive rate was 10.3%.
SIGNIFICANCE AND IMPACT OF THE STUDY
The high false-positive rate of Colilert-18, tempers its value for E. coli detection when used for tropical freshwater samples.
目的
评估Colilert - 18在检测热带淡水样本中的大肠杆菌和总大肠菌群方面的适用性。
方法与结果
分别使用m - TEC琼脂和m - ENDO LES琼脂从Colilert - 18/Quanti - Tray的黄色荧光孔和黄色孔中分离目标微生物。所有选定的分离株首先根据其脂肪酸甲酯谱进行鉴定。对与Colilert - 18检测结果矛盾的分离株，使用API 20E试纸条进行重新鉴定。共鉴定出357株分离株，其中177株来自黄色荧光孔，180株来自黄色孔。
结论
使用Colilert - 18检测大肠杆菌时的假阳性率和假阴性率分别为36.4%和11%，而检测大肠菌群时的假阳性率为10.3%。
研究的意义和影响
Colilert - 18的高假阳性率限制了其在检测热带淡水样本中大肠杆菌时的价值。
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