矮陀陀提取物对去卵巢大鼠体重和脂肪因子的调节作用

Labisia pumila extract regulates body weight and adipokines in ovariectomized rats.

作者信息

Fazliana Mansor, Wan Nazaimoon W M, Gu Harvest F, Ostenson Claes-Göran

机构信息

Department of Molecular Medicine and Surgery, M1:03, Karolinska Institutet, Karolinska University Hospital, SE-171 76 Stockholm, Sweden.

出版信息

Maturitas. 2009 Jan 20;62(1):91-7. doi: 10.1016/j.maturitas.2008.10.004. Epub 2008 Dec 2.

DOI:10.1016/j.maturitas.2008.10.004

PMID:19054635

Abstract

OBJECTIVE

We investigated the effects of a standardized water extract of Labisia pumila var. alata (LPva), and compared to estrogen replacement (ERT), on body weight gain, uterus weight, adipose tissue mRNA and protein levels of adipokines in ovariectomized (OVX) rats.

METHODS

Eight-week-old OVX Sprague-Dawley rats were administered orally with either 10 mg/kg/day (LPva10), 20 mg/kg/day (LPva20) or 50 mg/kg/day (LPva50) of LPva for 30 days. Sham-operated (Sham) and estrogen-treated OVX rats (ERT, 0.625 mg/kg/day) served as controls. Plasma adipokines were measured, and mRNA expressions of the adipokines were determined in the adipose tissues.

RESULTS

ERT- and LPva50-treated OVX rats showed significantly less (p<0.05) weight gain compared to untreated OVX rats. Ovariectomy caused plasma leptin levels to decrease significantly (p<0.05), but when treated with LPva or ERT, plasma leptin increased significantly to levels higher or comparable to that seen in the Sham group. The mRNA expression of leptin was higher in the LPva-treated animals than in all other groups. In contrast, the elevated plasma resistin concentrations in OVX rats were significantly reduced in rats given ERT (p<0.05) and LPva extracts (p<0.05). There was no difference in adiponectin levels in all groups. The uterus to body weight ratio of untreated OVX rats was significantly low compared to Sham (p<0.05), but showed dose-dependent increase upon treatment with LPva.

CONCLUSION

The present study provides first evidence that LPva exerts uterotrophic effect and regulates body weight gain by modulating secretion of leptin and resistin, and expression of the adipokines in adipose tissues.

摘要

目的

我们研究了标准化的翼叶美登木水提取物（LPva）对去卵巢（OVX）大鼠体重增加、子宫重量、脂肪组织中脂肪因子的mRNA和蛋白质水平的影响，并与雌激素替代疗法（ERT）进行比较。

方法

8周龄的OVX斯普拉格-道利大鼠连续30天口服10mg/kg/天（LPva10）、20mg/kg/天（LPva20）或50mg/kg/天（LPva50）的LPva。假手术（Sham）和雌激素处理的OVX大鼠（ERT，0.625mg/kg/天）作为对照。测定血浆脂肪因子，并在脂肪组织中测定脂肪因子的mRNA表达。

结果

与未处理的OVX大鼠相比，ERT和LPva50处理的OVX大鼠体重增加显著减少（p<0.05）。卵巢切除术导致血浆瘦素水平显著降低（p<0.05），但用LPva或ERT处理后，血浆瘦素显著增加至高于或与假手术组相当的水平。LPva处理的动物中瘦素的mRNA表达高于所有其他组。相比之下，ERT处理的大鼠（p<0.05）和LPva提取物处理的大鼠（p<0.05）中，OVX大鼠血浆抵抗素浓度的升高显著降低。所有组中脂联素水平无差异。与假手术组相比，未处理的OVX大鼠子宫与体重之比显著较低（p<0.05），但用LPva处理后呈剂量依赖性增加。