Iriti Marcello, Di Maro Antimo, Bernasconi Silvana, Burlini Nedda, Simonetti Paolo, Picchi Valentina, Panigada Cinzia, Gerosa Giacomo, Parente Augusto, Faoro Franco
Istituto di Patologia Vegetale, Universita di Milano, Milano, Italy.
J Agric Food Chem. 2009 Jan 14;57(1):201-8. doi: 10.1021/jf802819m.
The effect of chronic exposure to ozone pollution on nutritional traits of bean ( Phaseolus vulgaris L. cv. Borlotto Nano Lingua di Fuoco) seeds from plants grown in filtered and nonfiltered open-top chambers (OTCs) has been investigated. Results showed that, among seed macronutrients, ozone significantly raised total lipids, crude proteins, and dietary fiber and slightly decreased total free amino acid content, although with a significant reduction of asparagine, lysine, valine, methionine, and glycine, compensated by a conspicuous augmentation of ornithine and tryptophan. Phytosterol analysis showed a marked increase of beta-sitosterol, stigmasterol, and campesterol in seeds collected from nonfiltered OTCs. With regard to secondary metabolites, ozone exposure induced a slight increase of total polyphenol content, although causing a significant reduction of some flavonols (aglycone kaempferol and its 3-glucoside derivative) and hydroxycinnamates (caffeic, p-coumaric, and sinapic acids). Total anthocyanins decreased significantly, too. Nevertheless, ozone-exposed seeds showed higher antioxidant activity, with higher Trolox equivalent antioxidant capacity (TEAC) values than those measured in seeds collected from filtered air.
研究了长期暴露于臭氧污染对在过滤和未过滤的开顶式气室（OTC）中种植的菜豆（菜豆属普通菜豆品种Borlotto Nano Lingua di Fuoco）种子营养特性的影响。结果表明，在种子常量营养素中，臭氧显著提高了总脂质、粗蛋白和膳食纤维含量，并略微降低了总游离氨基酸含量，尽管天冬酰胺、赖氨酸、缬氨酸、蛋氨酸和甘氨酸显著减少，但鸟氨酸和色氨酸的显著增加起到了补偿作用。植物甾醇分析表明，从未过滤的OTC中收集的种子中β-谷甾醇、豆甾醇和菜油甾醇显著增加。关于次生代谢物，臭氧暴露导致总多酚含量略有增加，尽管一些黄酮醇（苷元山奈酚及其3-葡萄糖苷衍生物）和羟基肉桂酸（咖啡酸、对香豆酸和芥子酸）显著减少。总花青素含量也显著下降。然而，暴露于臭氧的种子显示出更高的抗氧化活性，其Trolox等效抗氧化能力（TEAC）值高于从过滤空气中收集的种子。
