Moon Hyung-In, Lee Jai-Heon
Department of Medicinal Biotechnology, College of Natural Resources and Life Science, Dong-A University, Busan 604-714, Korea.
Int J Mol Sci. 2012;13(8):9642-9648. doi: 10.3390/ijms13089642. Epub 2012 Aug 2.
To examine the neuroprotective effects of Glycine max, we tested its protection against the glutamate-induced toxicity in primary cortical cultured neurons. In order to clarify the neuroprotective mechanism(s) of this observed effect, isolation was performed to seek and identify active fractions and components. From such fractionation, two triterpene glycosides, 3-O-[α-l-rhamnopyranosyl(1-2)-β-d-glucopyranosyl(1-2)-β-d-glucuronopyranosyl]olean-12-en-3β,22β,24-triol (1) and 3-O-[β-d-glucopyranosyl(1-2)-β-d-galactopyranosyl(1-2)-β-d-glucuronopyranosyl]olean-12-en-3β,22β,24-triol (2) were isolated with the methanol extracts with of air-dried Glycine max. Among these compounds, compound 2 exhibited significant neuroprotective activities against glutamate-induced toxicity, exhibiting cell viability of about 50% at concentrations ranging from 0.1 μM to 10 μM. Therefore, the neuroprotective effect of Glycine max might be due to the inhibition of glutamate-induced toxicity by triterpene glycosides.
为了研究大豆的神经保护作用，我们测试了其对原代皮层培养神经元中谷氨酸诱导毒性的保护作用。为了阐明这种观察到的效应的神经保护机制，进行了分离以寻找和鉴定活性组分和成分。通过这种分级分离，从风干大豆的甲醇提取物中分离出两种三萜糖苷，3-O-[α-L-鼠李糖基(1-2)-β-D-葡萄糖基(1-2)-β-D-葡萄糖醛酸基]齐墩果-12-烯-3β,22β,24-三醇(1)和3-O-[β-D-葡萄糖基(1-2)-β-D-半乳糖基(1-2)-β-D-葡萄糖醛酸基]齐墩果-12-烯-3β,22β,24-三醇(2)。在这些化合物中，化合物2对谷氨酸诱导的毒性表现出显著的神经保护活性，在0.1 μM至10 μM的浓度范围内表现出约50%的细胞活力。因此，大豆的神经保护作用可能是由于三萜糖苷对谷氨酸诱导毒性的抑制作用。
