构建scrA::lacZ基因融合体以研究变形链球菌蔗糖磷酸转移酶系统的调控。
Construction of scrA::lacZ gene fusions to investigate regulation of the sucrose PTS of Streptococcus mutans.
作者信息
Sato Y, Yamamoto Y, Suzuki R, Kizaki H, Kuramitsu H K
机构信息
Department of Biochemistry, Tokyo Dental College, Chiba, Japan.
出版信息
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):339-45. doi: 10.1111/j.1574-6968.1991.tb04552.x.
The scrA gene coding for sucrose EnzymeII of the phosphoenolpyruvate dependent phosphotransferase system previously isolated from Streptococcus mutans was fused in vitro to the promoterless lacZ' gene to monitor the expression of the scrA gene. The scrA::lacZ gene fusion was introduced back into S. mutans GS-5IS3 by two independent transformation procedures involving either linear or plasmid DNA to produce both scrA and scrA+ mutants. These mutants should prove useful for analyzing the regulation of sucrose transport in S. mutans.
先前从变形链球菌中分离出的编码磷酸烯醇丙酮酸依赖性磷酸转移酶系统中蔗糖酶II的scrA基因，在体外与无启动子的lacZ'基因融合，以监测scrA基因的表达。通过涉及线性或质粒DNA的两种独立转化程序，将scrA::lacZ基因融合体重新导入变形链球菌GS-5IS3，以产生scrA和scrA+突变体。这些突变体应有助于分析变形链球菌中蔗糖转运的调控。
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