Amjad M, Castro J M, Sandoval H, Wu J J, Yang M, Henner D J, Piggot P J
Department of Microbiology and Immunology, Temple University School of Medicine, Philadelphia, PA 19140.
Gene. 1991 May 15;101(1):15-21. doi: 10.1016/0378-1119(91)90219-2.
A restriction map of 24 SfiI (GGCCN4/NGGCC) restriction fragments has been constructed for the Bacillus subtilis genome. The combined sizes of the fragments indicate a genome size of approx. 4.2 Mb. The SfiI fragments range in size from 7-730 kb. Genetic markers have been located on 19 of the SfiI fragments, and 69 genetic markers have been assigned to the SfiI restriction map.