克隆于酵母人工染色体中的枯草芽孢杆菌DNA片段的有序集合。
An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.
作者信息
Azevedo V, Alvarez E, Zumstein E, Damiani G, Sgaramella V, Ehrlich S D, Serror P
机构信息
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
出版信息
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6047-51. doi: 10.1073/pnas.90.13.6047.
Abstract
A collection of 772 Bacillus subtilis DNA segments was obtained by cloning in yeast artificial chromosomes. The B. subtilis inserts of 288 clones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in four contigs that cover > 98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.
摘要
通过酵母人工染色体克隆获得了772个枯草芽孢杆菌DNA片段的集合。使用65个克隆基因和188个分离的插入末端作为探针，通过杂交对288个克隆的枯草芽孢杆菌插入片段进行了定位。通过这种方式，59个插入片段被排列在四个重叠群中，覆盖了> 98%的枯草芽孢杆菌染色体。这个有序的集合现在可用于枯草芽孢杆菌基因组的进一步遗传和物理分析。
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