用半抗原免疫后，各种佐剂对鸡、绵羊和兔特异性抗体合成的影响。

Influence of various adjuvants on the synthesis of specific antibodies of chicken, sheep and rabbit following immunization with an hapten.

作者信息

Erhard M, Kellner J, Kühlmann R, Lösch U

机构信息

Institut für Physiologie, Physiologische Chemie und Ernährungsphysiologie, Tierärztliche Fakultät, Universität München, Germany.

出版信息

Zentralbl Veterinarmed A. 1991 Feb;38(1):21-7. doi: 10.1111/j.1439-0442.1991.tb00979.x.

DOI:10.1111/j.1439-0442.1991.tb00979.x

PMID:1905862

Abstract

In order to obtain high amounts of specific antibodies against the hapten methylphosphoric acid, para-aminophenyl-1,2,2-trimethyl-propyldiester (MATP) different animals were immunized with MATP coupled to the carrier protein human serum albumin (MATP12-HSA) using several adjuvants. The best specific immune response in sheep, rabbit and chicken was reached with Freund's complete adjuvant with animal specific differences being tested by an ELISA. The adjuvants aluminium hydroxide (5% and 10%) and diphosphoryl lipid A showed no significant difference compared to the control group (NaCl with MATP12-HSA). In rabbits and chickens MATP12-HSA can be used to reach an immune response without the help of an adjuvant.

摘要

为了获得大量针对半抗原甲基磷酸对氨基苯基 - 1,2,2 - 三甲基丙酯（MATP）的特异性抗体，使用几种佐剂，将与载体蛋白人血清白蛋白偶联的MATP（MATP12 - HSA）免疫不同的动物。通过酶联免疫吸附测定（ELISA）测试动物特异性差异，结果显示弗氏完全佐剂在绵羊、兔子和鸡中引发了最佳的特异性免疫反应。与对照组（含MATP12 - HSA的氯化钠）相比，氢氧化铝（5％和10％）和二磷酸脂质A佐剂未显示出显著差异。在兔子和鸡中，MATP12 - HSA无需佐剂的帮助即可引发免疫反应。