McWilliam A S, Vogel P
Animal Health Division, Western Australian Department of Agriculture, South Perth.
Vet Immunol Immunopathol. 1988 Jul;19(1):21-30. doi: 10.1016/0165-2427(88)90043-8.
Attempts were made to raise antibodies against corynetoxins, a family of toxins responsible for annual ryegrass toxicity. The glycolipid nature of corynetoxins made them ideally suited for incorporation into the structure of small unilamellar liposomes. Sheep were injected with corynetoxin liposomes with and without adjuvants such as lipid A and muramyl dipeptide, and the sera tested for anti-corynetoxin antibody. Similarly, rabbits were injected with hydrolysed corynetoxin coupled to human IgG and keyhole limpet haemocyanin and with corynetoxin coupled to bovine serum albumin. These preparations were administered with complete Freund's adjuvant. The failure of any of these preparations to elicit an anti-corynetoxin antibody response in either sheep or rabbits is discussed.
人们尝试制备抗棒杆菌毒素的抗体，棒杆菌毒素是一类导致一年生黑麦草中毒的毒素。棒杆菌毒素的糖脂性质使其非常适合整合到小单层脂质体结构中。给绵羊注射含有和不含有脂质A和胞壁酰二肽等佐剂的棒杆菌毒素脂质体，并检测血清中的抗棒杆菌毒素抗体。同样，给兔子注射与人免疫球蛋白和钥孔戚血蓝蛋白偶联的水解棒杆菌毒素以及与牛血清白蛋白偶联的棒杆菌毒素。这些制剂与完全弗氏佐剂一起给药。本文讨论了这些制剂在绵羊或兔子中均未能引发抗棒杆菌毒素抗体反应的情况。
