Colgrave Michelle L, Kotze Andrew C, Kopp Steven, McCarthy James S, Coleman Glen T, Craik David J
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia.
Acta Trop. 2009 Feb;109(2):163-6. doi: 10.1016/j.actatropica.2008.11.003. Epub 2008 Nov 18.
Hookworm infection is a leading cause of maternal and child morbidity in countries of the tropics and subtropics, as well as being an important parasite in companion-animal medicine. The cyclotides are a novel family of cyclic cystine knot containing peptides from plants that have been shown to possess anthelmintic activity against Haemonchus contortus and Trichostrongylus colubriformis, two important gastrointestinal nematodes of sheep. In the current study we demonstrated the in vitro effects of three representative cyclotides, kalata B1, kalata B6 and cycloviolacin O14, on the viability of larval and adult life stages of the dog hookworm Ancylostoma caninum, and larvae of the human hookworm Necator americanus. The cyclotides showed significant anthelmintic activity towards both hookworm species. The different cyclotides showed similar patterns of relative activity as that seen previously with the livestock nematode species. This study demonstrates that cyclotides have promising activity in vitro against important parasites of companion animals and humans.
钩虫感染是热带和亚热带国家孕产妇和儿童发病的主要原因，也是伴侣动物医学中的一种重要寄生虫。环肽是一类来自植物的新型含环胱氨酸结的肽，已被证明对捻转血矛线虫和蛇形毛圆线虫具有驱虫活性，这两种线虫是绵羊重要的胃肠道线虫。在本研究中，我们展示了三种代表性环肽kalata B1、kalata B6和环紫罗兰素O14对犬钩虫犬钩口线虫幼虫和成虫阶段以及人钩虫美洲板口线虫幼虫活力的体外影响。环肽对两种钩虫均表现出显著的驱虫活性。不同的环肽表现出与之前在牲畜线虫物种中观察到的相似的相对活性模式。本研究表明，环肽在体外对伴侣动物和人类的重要寄生虫具有有前景的活性。
