Aerts An M, Zabrocki Piotr, Govaert Gilmer, Mathys Janick, Carmona-Gutierrez Didac, Madeo Frank, Winderickx Joris, Cammue Bruno P A, Thevissen Karin
Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium.
FEBS Lett. 2009 Jan 5;583(1):113-7. doi: 10.1016/j.febslet.2008.11.028. Epub 2008 Dec 4.
We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological aging as compared to WT. Expression of genes encoding subunits of mitochondrial electron transport chain and ATP synthase is significantly downregulated in HsTnII, and as a consequence, HsTnII is not able to respire ethanol. All these data confirm the importance of functional mitochondria and respiration in determining yeast chronological lifespan and apoptosis.
我们之前分离出了一株酿酒酵母突变体（HsTnII），与野生型（WT）相比，其衰老寿命缩短了40%。在本研究中，我们发现HsTnII培养物的特征是线粒体碎片化且功能失调，与WT相比，在衰老过程中凋亡起始增加。线粒体电子传递链和ATP合酶亚基编码基因的表达在HsTnII中显著下调，因此，HsTnII无法利用乙醇进行呼吸。所有这些数据证实了功能性线粒体和呼吸作用在决定酵母衰老寿命和凋亡中的重要性。
