Mindlin S Z, Gorlenko Zh M, Bass I A, Khachikian N A
Genetika. 1990 Oct;26(10):1729-39.
The transfer of chromosomal and plasmid genes was studied via spontaneous transformation is mixed cultures of Acinetobacter spp. It turned out that any Acinetobacter strain, irrespective of its species specificity, serves as chromosomal DNA donor in case the mixed culture contains competent cells of the recipient strain. No transfer took place when non-related bacteria were used as donors. We also studied the transfer into Ac. calcoaceticus competent strain cells of small non-conjugative plasmids having broad host range (RSF1010, pAK1). In these cases, DNA donors could be not only acinetobacters of other species, but bacteria belonging to other systematic groups (families)--E. coli and P. aeruginosa. The transfer of plasmids from cells of unrelated bacteria took place with a frequency of about 10(-5)-10(-6). The possible role of spontaneous transformation in horizontal gene transfer is discussed.
通过不动杆菌属混合培养物中的自发转化来研究染色体和质粒基因的转移。结果表明，在混合培养物中含有受体菌株的感受态细胞的情况下，任何不动杆菌菌株，无论其种属特异性如何，都可作为染色体DNA供体。当使用非亲缘关系的细菌作为供体时，不会发生转移。我们还研究了具有广泛宿主范围的小型非接合质粒（RSF1010、pAK1）向乙酸钙不动杆菌感受态菌株细胞的转移。在这些情况下，DNA供体不仅可以是其他种的不动杆菌，还可以是属于其他系统类群（科）的细菌——大肠杆菌和铜绿假单胞菌。来自非亲缘关系细菌细胞的质粒转移频率约为10^(-5)-10^(-6)。文中讨论了自发转化在水平基因转移中的可能作用。
