通过基因组DNA电穿孔构建农杆菌菌株及其在染色体毒力突变分析中的应用。
Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.
作者信息
Charles T C, Doty S L, Nester E W
机构信息
Department of Microbiology, University of Washington, Seattle 98195.
出版信息
Appl Environ Microbiol. 1994 Nov;60(11):4192-4. doi: 10.1128/aem.60.11.4192-4194.1994.
Abstract
We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.
摘要
我们已经扩展了电穿孔技术，将其作为一种用于操纵根癌土壤杆菌染色体的遗传工具。我们使用该技术通过电穿孔将染色体DNA导入受体根癌土壤杆菌菌株，并构建了同基因的chvE突变体，这些突变体具有相同的染色体背景，但携带的Ti质粒类型（章鱼碱型或胭脂碱型）不同。携带胭脂碱型和章鱼碱型Ti质粒的chvE突变体在vir调控子诱导方面均存在缺陷，并且在宿主范围上表现出相似程度的降低。
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Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.通过基因组DNA电穿孔构建农杆菌菌株及其在染色体毒力突变分析中的应用。
Appl Environ Microbiol. 1994 Nov;60(11):4192-4. doi: 10.1128/aem.60.11.4192-4194.1994.
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Appl Environ Microbiol. 1993 May;59(5):1310-7. doi: 10.1128/aem.59.5.1310-1317.1993.
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Control of expression of Agrobacterium vir genes by synergistic actions of phenolic signal molecules and monosaccharides.通过酚类信号分子和单糖的协同作用控制农杆菌vir基因的表达。
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VirA, the plant-signal receptor, is responsible for the Ti plasmid-specific transfer of DNA to maize by Agrobacterium.VirA作为植物信号受体，负责农杆菌将DNA特异性转移至玉米的Ti质粒。
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A chromosomally encoded two-component sensory transduction system is required for virulence of Agrobacterium tumefaciens.根癌土壤杆菌的毒力需要一种由染色体编码的双组分传感转导系统。
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Genetics for all bacteria.所有细菌的遗传学
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The chromosomal virulence gene, chvE, of Agrobacterium tumefaciens is regulated by a LysR family member.根癌土壤杆菌的染色体毒力基因chvE受一个LysR家族成员调控。
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7
Design and development of amplifiable broad-host-range cloning vectors: analysis of the vir region of Agrobacterium tumefaciens plasmid pTiC58.可扩增的广宿主范围克隆载体的设计与开发：根癌土壤杆菌质粒pTiC58的vir区域分析
Plasmid. 1984 Sep;12(2):111-8. doi: 10.1016/0147-619x(84)90057-x.
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Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.在冠瘿瘤中未检测到根癌土壤杆菌DNA和PS8噬菌体DNA。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672-6. doi: 10.1073/pnas.71.9.3672.
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virA and virG control the plant-induced activation of the T-DNA transfer process of A. tumefaciens.virA和virG控制植物诱导的根癌土壤杆菌T-DNA转移过程的激活。
Cell. 1986 Aug 1;46(3):325-33. doi: 10.1016/0092-8674(86)90653-7.
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