Yassin A F, Siering C
Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, Bonn, Germany.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2876-9. doi: 10.1099/ijs.0.2008/000414-0.
A coryneform bacterium isolated from the sputum of a patient with pneumonia was characterized by phenotypic and molecular taxonomic methods. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV and short chain mycolic acids consistent with the genus Corynebacterium. Comparative 16S rRNA gene sequencing studies confirmed this assignment, with the organism forming a hitherto unknown subline within the genus associated with a subcluster containing Corynebacterium hansenii, Corynebacterium freneyi, Corynebacterium xerosis, Corynebacterium amycolatum and Corynebacterium sphenisci. Sequence divergence values of >2.7 % from established corynebacterial species suggested that the new isolate represented a novel species. This was also supported by the results of the biochemical tests. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown bacterium be classified as a novel species of the genus Corynebacterium, Corynebacterium sputi sp. nov. (type strain IMMIB L-999(T)=DSM 45148(T)=CCUG 55795(T)).
从一名肺炎患者痰液中分离出的一株棒状杆菌，通过表型和分子分类学方法进行了鉴定。化学分类学研究显示存在细胞壁化学型IV和短链分枝菌酸，这与棒状杆菌属相符。比较16S rRNA基因测序研究证实了这一归类，该菌株在棒状杆菌属内形成了一个迄今未知的亚系，与包含汉森棒状杆菌、弗雷内棒状杆菌、干燥棒状杆菌、无枝菌酸棒状杆菌和企鹅棒状杆菌的一个亚簇相关。与已确定的棒状杆菌物种的序列差异值>2.7%，表明新分离株代表一个新物种。生化试验结果也支持了这一点。基于表型和系统发育证据，建议将该未知细菌归类为棒状杆菌属的一个新物种，即痰液棒状杆菌（Corynebacterium sputi sp. nov.）（模式菌株IMMIB L-999(T)=DSM 45148(T)=CCUG 55795(T)）。
