Lee Hee Joong, Cho Sung-Lim, Jung Min Young, Van Nguyen Thi Hai, Jung Yeon-Chang, Park Hee Kuk, Le Van Phan, Kim Wonyong
Department of Microbiology and Research Institute for Translational System Biomics, Chung-Ang University College of Medicine, Dongjak-gu, Seoul 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2734-7. doi: 10.1099/ijs.0.010108-0. Epub 2009 Jul 22.
The taxonomic position of a Gram-positive, non-motile, non-spore-forming coryneform, isolated from activated sludge and designated strain CAU 212(T), was investigated using a polyphasic approach. Cellular morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 212(T) had the characteristics of the genus Corynebacterium. Comparative 16S rRNA gene sequence analysis showed that the organism formed a hitherto-unknown subline within the genus Corynebacterium. Sequence divergence values of more than 4.3 % from recognized Corynebacterium species, together with phenotypic differences, showed that the bacterium represents a previously unrecognized member of the genus Corynebacterium, for which the name Corynebacterium doosanense sp. nov. is proposed. The type strain is CAU 212(T) (=KCTC 19568(T)=CCUG 57284(T)).
采用多相分类法对从活性污泥中分离得到的一株革兰氏阳性、无运动性、不产芽孢的棒状杆菌（命名为菌株CAU 212(T)）的分类地位进行了研究。细胞形态学、生化试验和化学分类学研究表明，菌株CAU 212(T)具有棒状杆菌属的特征。16S rRNA基因序列比较分析表明，该菌在棒状杆菌属内形成了一个迄今未知的亚系。与已确认的棒状杆菌属物种的序列差异值超过4.3%，以及表型差异，表明该细菌代表了棒状杆菌属中一个先前未被认识的成员，为此提出新种名为斗山棒状杆菌（Corynebacterium doosanense sp. nov.）。模式菌株为CAU 212(T)（=KCTC 19568(T)=CCUG 57284(T)）。
