Baik Keun Sik, Park Seong Chan, Kim Mi Sun, Kim Eun Mi, Park Chul, Chun Jongsik, Seong Chi Nam
Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon, Republic of Korea.
Int J Syst Evol Microbiol. 2008 Dec;58(Pt 12):2904-8. doi: 10.1099/ijs.0.2008/000281-0.
A marine bacterial strain, designated FR1330(T), was isolated from a seawater sample collected near Ganghwa Island, the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FR1330(T) belonged to the Gammaproteobacteria and was related to the genus Luteimonas; its closest neighbours were the type strains of Luteimonas composti (97.9 % sequence similarity) and Luteimonas mephitis (95.0 %). DNA-DNA relatedness values for strain FR1330(T) with Luteimonas composti CC-YY255(T) and Luteimonas mephitis KACC 11391(T) were 33 and 10 %, respectively. Cells of strain FR1330(T) were Gram-negative, aerobic, rod-shaped and oxidase- and catalase-positive. The predominant respiratory lipoquinone was ubiquinone-8. The major fatty acids were branched-chain saturated iso-C(15 : 0) (26.2 %) and unsaturated iso-C(17 : 1)omega9c (26.0 %). The DNA G+C content was 67.6 mol%. On the basis of several phenotypic characteristics, strain FR1330(T) could be differentiated from Luteimonas composti and Luteimonas mephitis. The data obtained from the polyphasic study demonstrated clearly that strain FR1330(T) represents a novel species of the genus Luteimonas. The name Luteimonas marina sp. nov. is proposed, with strain FR1330(T) (=KCTC 12327(T)=JCM 12488(T)=IMSNU 60306(T)) as the type strain.
从韩国江华岛附近采集的海水样本中分离出一株海洋细菌，命名为FR1330(T)。基于16S rRNA基因序列的系统发育分析表明，菌株FR1330(T)属于γ-变形菌纲，与藤黄单胞菌属相关；其最接近的亲缘种是堆肥藤黄单胞菌（序列相似性为97.9%）和恶臭藤黄单胞菌（序列相似性为95.0%）的模式菌株。菌株FR1330(T)与堆肥藤黄单胞菌CC-YY255(T)和恶臭藤黄单胞菌KACC 11391(T)的DNA-DNA杂交值分别为33%和10%。菌株FR1330(T)的细胞为革兰氏阴性、需氧、杆状，氧化酶和过氧化氢酶呈阳性。主要呼吸醌为泛醌-8。主要脂肪酸为支链饱和异-C(15 : 0)（26.2%）和不饱和异-C(17 : 1)ω9c（26.0%）。DNA G+C含量为67.6 mol%。基于几个表型特征，菌株FR1330(T)可与堆肥藤黄单胞菌和恶臭藤黄单胞菌区分开来。多相研究获得的数据清楚地表明，菌株FR1330(T)代表藤黄单胞菌属的一个新物种。提出新种名海洋藤黄单胞菌（Luteimonas marina sp. nov.），以菌株FR1330(T)（=KCTC 12327(T)=JCM 12488(T)=IMSNU 60306(T)）作为模式菌株。
