鉴定用于逆向代谢工程的改良木糖发酵酿酒酵母中的共同特征。
Identification of common traits in improved xylose-growing Saccharomyces cerevisiae for inverse metabolic engineering.
作者信息
Bengtsson Oskar, Jeppsson Marie, Sonderegger Marco, Parachin Nadia Skorupa, Sauer Uwe, Hahn-Hägerdal Bärbel, Gorwa-Grauslund Marie-F
机构信息
Department of Applied Microbiology, Lund University, PO Box 124, 221 00 Lund, Sweden.
出版信息
Yeast. 2008 Nov;25(11):835-47. doi: 10.1002/yea.1638.
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome-wide transcription analysis in order to identify novel targets for inverse metabolic engineering. A subset of 13 genes with changed expression levels in all improved strains was selected for further analysis. Thirteen validation strains and two reference strains were constructed to investigate the effect of overexpressing or deleting these genes in xylose-utilizing S. cerevisiae. Improved aerobic growth rates on xylose were observed in five cases. The strains overexpressing SOL3 and TAL1 grew 19% and 24% faster than their reference strain, and the strains carrying deletions of YLR042C, MNI1 or RPA49 grew 173%, 62% and 90% faster than their reference strain.
通过全基因组转录分析，将四株木糖生长增强的重组酿酒酵母菌株（TMB3400、C1、C5和BH42）与两株对照菌株（TMB3399、TMB3001）进行比较，以确定逆向代谢工程的新靶点。选择了在所有改良菌株中表达水平发生变化的13个基因的一个子集进行进一步分析。构建了13个验证菌株和两个参考菌株，以研究在利用木糖的酿酒酵母中过表达或缺失这些基因的效果。在五个案例中观察到木糖上有氧生长速率提高。过表达SOL3和TAL1的菌株比其参考菌株生长快19%和24%，而缺失YLR042C、MNI1或RPA49的菌株比其参考菌株生长快173%、62%和90%。
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