Patwardhan S, Gashler A, Siegel M G, Chang L C, Joseph L J, Shows T B, Le Beau M M, Sukhatme V P
Department of Medicine, Molecular Genetics and Cell Biology, Howard Hughes Medical Institute, University of Chicago, Illinois 60637.
Oncogene. 1991 Jun;6(6):917-28.
We have previously described two cellular immediate-early genes, Egr-1 (mouse) and EGR2 (human) that encode zinc finger proteins. Here we report the characterization of a new member of the Egr family referred to as EGR3 (human). This cDNA clone was isolated using low stringency hybridization with the zinc finger domain of Egr-1. The EGR3 cDNA sequence predicts a 387 amino acid (a.a.) protein containing three Cys2-His2 zinc fingers nearly identical to those of Egr-1 and EGR2. This similarity has a functional consequence: EGR3 can activate transcription of a CAT gene linked to the sequence CGCCCCCGC, a cis element which is a target for Egr-1 and EGR2. We show that EGR3 is an immediate-early growth response gene induced by mitogenic stimulation of rodent and human fibroblasts and a monkey kidney epithelial cell line. The EGR3 gene has a single intron and maps to chromosome 8 at bands p21-23.
我们之前描述过两个细胞即刻早期基因，即编码锌指蛋白的Egr-1（小鼠）和EGR2（人类）。在此我们报告Egr家族一个新成员EGR3（人类）的特征。该cDNA克隆是利用与Egr-1锌指结构域的低严谨度杂交分离得到的。EGR3 cDNA序列预测编码一个含387个氨基酸的蛋白质，该蛋白质含有三个Cys2-His2锌指，与Egr-1和EGR2的锌指几乎相同。这种相似性具有功能上的影响：EGR3能够激活与序列CGCCCCCGC相连的CAT基因的转录，该顺式元件是Egr-1和EGR2的作用靶点。我们发现EGR3是一种即刻早期生长反应基因，在啮齿动物和人类成纤维细胞以及猴肾上皮细胞系的促有丝分裂刺激下被诱导表达。EGR3基因有一个内含子，定位于8号染色体的p21-23带。
