Doan Thuy T P, Carlsson Anders S, Hamberg Mats, Bülow Leif, Stymne Sten, Olsson Peter
Department of Plant Breeding and Biotechnology, Swedish University of Agricultural Sciences, P.O. Box 101, 23053 Alnarp, Sweden.
J Plant Physiol. 2009 May 15;166(8):787-96. doi: 10.1016/j.jplph.2008.10.003. Epub 2008 Dec 4.
Very long chain primary alcohols are significant components in cuticle waxes of plants. Fatty acyl-CoA reductases (FARs) catalyze the formation of a fatty alcohol from an acyl-CoA. The Arabidopsis (Arabidopsis thaliana) genome contains eight genes homologous to FAR genes from jojoba (Simmondsia chinensis), silk moth, wheat and mouse. Expression of six Arabidopsis FAR homologs in Escherichia coli resulted in production of alcohols from endogenous E. coli fatty acids by five of these genes, confirming that they encode for FAR enzymes. Only a truncated splicing version of the sixth gene was found, and this gene yielded a protein with no FAR activity. The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.
极长链伯醇是植物角质层蜡中的重要成分。脂肪酰辅酶A还原酶（FARs）催化由酰基辅酶A形成脂肪醇。拟南芥（Arabidopsis thaliana）基因组包含八个与来自霍霍巴（Simmondsia chinensis）、家蚕、小麦和小鼠的FAR基因同源的基因。六个拟南芥FAR同源物在大肠杆菌中的表达导致其中五个基因利用大肠杆菌内源性脂肪酸产生了醇，证实它们编码FAR酶。仅发现了第六个基因的一个截短的剪接版本，该基因产生的蛋白质没有FAR活性。当在大肠杆菌中表达时，这五种有功能的FAR酶产生了明显不同的脂肪醇组成，表明不同的酶可能参与植物细胞中不同类型醇的产生。
