Aston K I, Li G P, Hicks B A, Sessions B R, Davis A P, Winger Q A, Rickords L F, Stevens J R, White K L
Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, Utah, USA.
Mol Reprod Dev. 2009 May;76(5):471-82. doi: 10.1002/mrd.20962.
Low developmental competence of bovine somatic cell nuclear transfer (SCNT) embryos is a universal problem. Abnormal placentation has been commonly reported in SCNT pregnancies from a number of species. The present study employed Affymetrix bovine expression microarrays to examine global gene expression patterns of SCNT and in vivo produced (AI) blastocysts as well as cotyledons from day-70 SCNT and AI pregnancies. SCNT and AI embryos and cotyledons were analyzed for differential expression. Also in an attempt to establish a link between abnormal gene expression patterns in early embryos and cotyledons, differentially expressed genes were compared between the two studies. Microarray analysis yielded a list of 28 genes differentially expressed between SCNT and AI blastocysts and 19 differentially expressed cotyledon genes. None of the differentially expressed genes were common to both groups, although major histocompatibility complex I (MHCI) was significant in the embryo data and approached significance in the cotyledon data. This is the first study to report global gene expression patterns in bovine AI and SCNT cotyledons. The embryonic gene expression data reported here adds to a growing body of data that indicates the common occurrence of aberrant gene expression in early SCNT embryos.
牛体细胞核移植（SCNT）胚胎发育能力低下是一个普遍存在的问题。在许多物种的SCNT妊娠中，胎盘异常的情况屡见不鲜。本研究采用Affymetrix牛表达微阵列技术，检测SCNT胚胎和体内受精（AI）胚胎囊胚以及第70天SCNT妊娠和AI妊娠的子叶的整体基因表达模式。对SCNT和AI胚胎及子叶进行差异表达分析。此外，为了试图建立早期胚胎和子叶中异常基因表达模式之间的联系，对两项研究中差异表达的基因进行了比较。微阵列分析得出了一份在SCNT和AI囊胚之间差异表达的28个基因的列表，以及19个差异表达的子叶基因。两组中没有差异表达的基因是相同的，尽管主要组织相容性复合体I（MHCI）在胚胎数据中具有显著性，在子叶数据中接近显著性。这是第一项报道牛AI和SCNT子叶整体基因表达模式的研究。这里报道的胚胎基因表达数据增加了越来越多的数据，表明早期SCNT胚胎中异常基因表达普遍存在。
